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| 利用农杆菌浸种法将叶片衰老抑制基因P_(SAG12)-IPT导入普通小麦的研究 | |
| 引用本文： | 奚亚军,张启发,林拥军,侯文胜,路明.利用农杆菌浸种法将叶片衰老抑制基因P_(SAG12)-IPT导入普通小麦的研究[J].中国农业科学,2004,37(8):1235-1235. |
| 作者姓名： | 奚亚军 张启发 林拥军 侯文胜 路明 |
| 作者单位： | 1. 华中农业大学/作物遗传改良国家重点实验室,武汉,430070;西北农林科技大学学院,杨凌,712100 2. 华中农业大学/作物遗传改良国家重点实验室,武汉,430070 3. 西北农林科技大学学院,杨凌,712100 |
| 基金项目： | 华中农业大学作物遗传改良国家重点实验室开放课题资助项目，国家重点科技(攻关)资助项目(96-009-01-14)，国家转基因专项资助项目(jy03-b-23-02) |
| 摘 要： | 以普通小麦品种西农1376和西农2611为材料,利用农杆菌浸种法获得1株导入了叶片衰老抑制基因PSAG12-IPT转基因植株。经PCR、GUS组织化学染色和Southern杂交分析,证明目的基因已整合到小麦基因组中并在转基因植株中能够稳定遗传。转基因小麦在叶片细胞分裂素(异戊烯基腺嘌呤)含量、叶片衰老进程及农艺性状方面与对照无明显差异,初步表明叶片衰老抑制基因PSAG12-IPT,在转基因植株体内可能未表达或表达量不足。 |
| 关 键 词： | 小麦 农杆菌浸种法 叶片衰老 P_(SAG12)-IPT |
Introduction of Leaf Senescence-Inhibiting Gene PSAG12-IPTinto Common Wheat via Agrobacterium tumefaciens Soaked Seeds |
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| XI Ya-jun,ZHANG Qi-fa,LIN Yong-jun,HOU Wen-sheng,LU Ming.Introduction of Leaf Senescence-Inhibiting Gene PSAG12-IPTinto Common Wheat via Agrobacterium tumefaciens Soaked Seeds[J].Scientia Agricultura Sinica,2004,37(8):1235-1235. | |
| Authors: | XI Ya-jun ZHANG Qi-fa LIN Yong-jun HOU Wen-sheng LU Ming |
| Abstract: | A transgenic plant with PSAG12-IPT gene was obtained by means of Agrobacterium tumefaciens soaked seeds from wheat Xinong1376. The integration of PSAG12-IPT gene into the wheat genome was confirmed by PCR amplification, GUS histochemical and Southern blot hybridization analysis and the PSAG12-IPT gene could be inherited stably. The investigation on leaf cytokinin (isopentenyladenine) content, leaf senescence development and agronomical characters showed that there were no significant difference between transgenic wheat and Xinong1376 . The result indicted that PSAG12-IPT gene might not express (gene silencing) or express partially in transgenic wheat plant. |
| Keywords: | Wheat Agrobacterium tumefaciens soaked seeds Leaf senescence PSAG12-IPT |
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