HPLC-MSMS测定白鲜皮中酮的含量

建立一种快速、准确测定白鲜皮中梣酮含量的HPLC-MSMS分析方法。测定方法为:液相色谱条件为XDB-C18柱,流动相为甲醇—水,m(甲醇)∶m(水)=65∶35,流量为0.3mL/min。质谱条件为Fragmento电压100V,定量离子m/z215,碰撞能量4V;定性离子m/z187,碰撞能量10V。结果表明,质量浓度为0.0084~0.840mg/L,相关性R2=0.99999,回收率92.8%~100.0%;定性检出浓度(S/N=3)0.002mg/L;定量检出浓度(S/N=10)0.007mg/L。该方法准确可靠,适用于白鲜皮中梣酮的定量分析。

Determination of Fraxinellone in Dictamnus Dasycarpus Turcz Using High Performance Liquid Chromatography-tandem Mass Spectrometry

An analyzing method based on HPLC-MSMS was established to determine the fraxinellone content in Dictamnus dasycarpus Turcz.fleetly and truly.Methods: The condition of liquid chromatogram: XDB-C18 column,mobile phase: methanol∶water=65∶35,flow rate:0.3 mL/min.The condition of mass spectrum:Fragmento voltage 110 V,quantificational ion m/z 215,collision energy 4 V;qualitative ion m/z 187,collision energy 10 V.Results:When the range of concentration was from 0.008 4 mg/L to 0.840 mg/L,relativity R2=0.999 99,the recoveries of this method in the range of 92.8%~100.0%.Qualitative concentration of detection (S/N=3):0.002 mg/L,quantificational concentration of detection (S/N=3):0.007 mg/L.Conclusion:The method was accurate.It can be applied to the determination of the fraxinellone content in Dictamnus dasycarpus Turcz patulin.