黄孢原毛平革菌后处理深度提升醋糟产甲烷潜力

木质素的脱除是醋糟厌氧消化性能提升的关键。以厌氧发酵后的醋糟为底物,利用黄孢原毛平革菌(Phanerochaete chrysosporium)后处理醋糟脱除木质素的方式,深度提升其产甲烷潜力,并考查不同后处理条件(接种量和后处理时间)下醋糟的降解情况和产甲烷潜力。实验结果表明:加大接种量和延长后处理时间可以促进P. chrysosporium木质纤维素酶的分泌,从而获得更佳的底物降解效果。当接种量为9块、后处理时间为16 d时(实验组T9-16),醋糟的降解效果最佳,总固体和挥发性固体的降解率分别为8.96%和9.91%,纤维素、半纤维素和木质素的降解率分别为7.84%、23.72%和11.84%。进一步的产甲烷潜力实验结果表明,P. chrysosporium后处理有效提高了醋糟的可生物降解性。接种量为6块、后处理时间为16 d的实验组(T6-16)产气最佳,单位质量底物(以挥发性固体计)甲烷产气量达到了246.8 mL·g^-1,是对照组(醋糟未进行后处理)的2.82倍。本研究证实了P. chrysosporium后处理提升醋糟厌氧消化产甲烷潜力的可行性。

Improvement of methane production potential by post-treatment of vinegar residue with Phanerochaete chrysosporium

Delignification is the key to improve the anaerobic digestion performance of vinegar residue. In this study, vinegar residue after anaerobic fermentation was used as substrate. The delignification was realized by post-treatment of vinegar residue with Phanerochaete chrysosporium, so as to deeply improve the methane production potential. The degradation and methane production potential of vinegar residue were investigated under different post-treatments (inoculation amount and post-treatment time). It was shown that increasing the inoculum amount and prolonging the post-treatment time could promote the secretion of lignocellulase, so as to obtain higher degradation effect. When the inoculation amount was 9 pieces and the post-treatment time was 16 days (experimental group T9-16), the substrate degradation efficiency was the highest. The degradation rates of total solids and violate solids were 8.96% and 9.91%, respectively. And the degradation rates of cellulose, hemicellulose and lignin reached 7.84%, 23.72% and 11.84%, respectively. Further experiments on the methane production potential showed that the biodegradability of vinegar residue was improved effectively by the post-treatment with P. chrysosporium. The optimal methane production was achieved in the experimental group T6-16 with 6 pieces of inoculation amount and 16 days of post-treatment time. The methane production per unit substrate (based on violate solids) of T6-16 reached up to 246.8 mL·g^-1, which was 2.82 times of that in the control group (vinegar residue without post-treatment). This study confirmed the feasibility of post-treatment with P. chrysosporium in promoting the anaerobic digestion efficiency of vinegar residue.