应用ISSR分子标记评价我国丝瓜种质资源遗传多样性

应用简单重复序列间扩增(ISSR)对73份丝瓜种质资源进行遗传多样性分析,从60条ISSR引物中筛选获得多态性引物15条,共扩增出99条DNA条带,平均每条引物扩增6.6条,多态性条带92条,非多态性条带7条,多态性比例在66.67%~100%,平均多态性比例为92.90%,种质间具有较高的遗传多样性,种质间遗传相似系数在0.454 5~0.959 6。通过UPGMA聚类分析可知,0.59为阈值时,分为2类,有棱丝瓜和普通丝瓜;0.66为阈值时,分为4类,分别是常山花斑普通丝瓜、绿色或淡绿色普通丝瓜、来源于浙西地区的短棍棒有棱丝瓜和来自两广地区的长棍棒有棱丝瓜。

Evaluation of genetic diversity in Luffa germplasm resources in China based on ISSR molecular marker

The genetic diversity of 73 Luffa germplasms was analyzed with inter-simple sequence repeat(ISSR) molecular markers. Fifteen pairs of ISSR primers were screened out of 60 ISSR primer pairs, which produced 99 DNA bands, the average number of polymorphic DNA bands amplified by each primer pair was 6.6, 92 polymorphic bands, 7 basic bands, the proportion of polymorphic primers ranged from 66.67% to 100% and the ratio of polymorphic bands was 92.90%.The results showed the high genetic diversity of Luffa germplasm resources. The value of Nei’s genetic similarity (GS) indexes of 73 Luffa germplasm varied from 0.454 5 to 0.959 6. Clutser analysis with UPGMA method showed that the 73 Luffa germplasm were clustered into 2 groups, Luffa cylindrica(L.) Roem and Luffa acutangula(L.) Roxb., with the similarity coefficient of 0.59. However, with the similarity coefficient of 0.66, these Luffa germplasm were clustered into 4 groups, including tinea fruit, green or light green fruit types, short stick types from west Zhejiang province and long stick types from Guangdong and Guangxi areas.