小对叶植株再生及遗传转化体系构建

为了利用生物技术方法开发及改良水生植物小对叶，以小对叶（Bacopa monnieri）叶片为外植体，建立其组织培养再生体系及根癌农杆菌介导的遗传转化体系。结果表明：小对叶叶盘最佳愈伤组织诱导培养基是MS+6-BA 0.5 mg/L+NAA 0.02 mg/L；最佳分化培养基是MS+6-BA 0.5 mg/L+NAA 0.05 mg/L；最佳生根培养基是1/2MS+IBA 0.1 mg/L。最佳的根癌农杆菌介导的小对叶遗传转化条件是：外植体预培养2天，侵染菌液OD600为0.5，侵染时间7 min、共培养4天和延迟筛选4天，可获得最高转化率21.4%。

Regeneration and Genetic Transformation of Aquatic Plants Bacopa monnieri

In order to explore and improve the aquatic plant Bacopa monnieri with biotechnological methods, regeneration from tissue culture and Agrobacterium-mediated genetic transformation system were established with leaves of Bacopa monnieri as explants. The results showed that the optimal callus induction medium was MS ＋ 6-BA 0.5 mg /L ＋ NAA 0.02 mg/L; differentiation medium was MS ＋ 6-BA 0.5 mg/L ＋ NAA 0.05 mg/L; rooting medium was 1/2MS ＋ IBA 0.1 mg/L. The best Agrobacterium tumefaciens-mediated genetic transformation conditions were as follows： explants pre-culturing for 2 days, OD 600 of infecting bacilli 0.5, infection for 7 min, co-culturing for 4 days and delay screening after 4 days. The highest frequency of transformation occurred in 21.4%.