| 不同耐草甘膦转基因大豆对根际土壤固氮细菌多样性的影响 |
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| 引用本文： | 章秋艳,赵建宁,李 刚,王丽娟,王 慧,常 泓,杨殿林.不同耐草甘膦转基因大豆对根际土壤固氮细菌多样性的影响[J].农业环境科学学报,2013,32(9):1827-1833. |
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| 作者姓名： | 章秋艳 赵建宁 李 刚 王丽娟 王 慧 常 泓 杨殿林 |
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| 作者单位： | 农业部环境保护科研监测所， 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点实验室；山西农业大学生命科学学院;农业部环境保护科研监测所， 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点实验室;农业部环境保护科研监测所， 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点实验室;农业部环境保护科研监测所， 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点实验室；内蒙古师范大学生命科学与技术学院;农业部环境保护科研监测所， 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点实验室;山西农业大学生命科学学院;农业部环境保护科研监测所， 农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点实验室 |
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| 摘 要： | 采用盆栽试验结合DGGE-cloning测序技术比较分析了耐草甘膦转基因大豆M88、GTS40-3-2、ZB及常规非转基因大豆中黄13在成熟期对根际土壤固氮细菌多样性的影响。结果表明，耐草甘膦转基因大豆M88、GTS40-3-2和非转基因大豆中黄13根际土壤固氮细菌多样性指数和均匀度指数无显著差异；抗虫耐草甘膦转基因大豆ZB根际土壤固氮细菌多样性指数显著高于非转基因大豆中黄13，均匀度指数无显著差异。克隆测序结果表明，4种大豆根际土壤固氮细菌主要隶属于α-变形菌纲、β-变形菌纲、γ-变形菌纲、蓝藻纲和一些未知细菌类。相对于不同大豆品种对固氮细菌多样性的影响，耐草甘膦CP4 epsps基因的导入并没有对根际土壤固氮细菌多样性产生显著影响。
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| 关 键 词： | 转基因大豆 根际土壤 固氮细菌 多样性 nifH DGGE-cloning |
Effects of Different Glyphosate-tolerance Soybeans on the Diversity of Nitrogen-fixing Bacteria in Rhizosphere Soil |
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| ZHANG Qiu-yan,ZHAO Jian-ning,LI Gang,WANG Li-juan,WANG Hui,CHANG Hong and YANG Dian-lin.Effects of Different Glyphosate-tolerance Soybeans on the Diversity of Nitrogen-fixing Bacteria in Rhizosphere Soil[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2013,32(9):1827-1833. |
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| Authors: | ZHANG Qiu-yan ZHAO Jian-ning LI Gang WANG Li-juan WANG Hui CHANG Hong and YANG Dian-lin |
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| Institution: | Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety;College of Life Science, Shanxi Agricultural University;Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety;Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety;Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety;College of Life Science and Technology, Inner Mongolia Normal University;Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety;College of Life Science, Shanxi Agricultural University;Agro-Environmental Protection Institute, Ministry of Agriculture, Open Fund of Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture and Tianjin Key Laboratory of Agro-environment and Agro-product Safety |
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| Abstract: | |
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| Keywords: | transgenic soybean rhizosphere soil nitrogen-fixing bacteria diversity nifH DGGE-cloning |
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