牛源犬新孢子虫IMP1基因的克隆与序列分析

目的]为了解牛源犬新孢子虫IMP1基因生物学特性,分析牛源犬新孢子虫IMP1基因的克隆与序列。方法]应用PCR技术扩增IMP1基因,将纯化的PCR产物和pMD18-T Simple Vector连接,构建pMD18-IMP1重组克隆质粒,进行PCR鉴定和酶切鉴定,将鉴定为阳性的质粒进行测序分析。结果]PCR扩增犬新孢子虫IMP1基因大小为762 bp,克隆质粒经测序分析与GenBank（XM003879531.1）中IMP1基因的同源性为99.9%。结论]该试验为犬新孢子虫IMP1基因的表达及后续研究奠定了基础。

Cloning and Sequence Analysis of Bovine Neospora caninum IMP1 Gene

Objective]In order to understand IMP1 genitival biological characteristics,analyze cloning and sequence of bovine Neospora caninum IMP1 gene. Method] PCR technology was applied for amplification of IMP1 genes. The products of purified PCR were connected with pMD18-T Simple Vector to construct the pMD18-IMP1 cloned plasmid,then PCR identification and enzyme digestion identification of recombinant plasmid were conducted. The positive plasmid was identified by sequencing analysis. Result]Neospora caninum IMP1 amplified by PCR is 762 bp,the analysis of sequencing results shows that the GenBank（ XM_003879531. 1） IMP1 is 99% in gene homology. Conclusion] The method laid a foundation for expression and subsequent research of Neospora caninum IMP1 gene.