黑莓的组织培养快速繁殖技术

以黑莓茎尖和带芽茎段为外植体,筛选组织培养各阶段适宜培养基.结果表明:外植体以茎段为宜.初始培养基以MS BA 1.0 mg/L IAA 0.1 mg/L最好,出芽率达93%;继代增殖培养基以MS BA 1.0 mg/L NAA 0.05～0.2 mg/L最理想,增殖系数可达8倍;生根培养基以1/2 MS IBA0.1 mg/L和1/2 MS NAA0.05 mg/L最佳,生根率可达100%.以园土:珍珠岩:蚯蚓粪按1:1:1的比例混合的基质移栽成活率达到96.9%,移栽苗生长健壮.

Study on Tissue Culture and Rapid Propagation of Blackberry

Using stem segments with buds as explants,tissue culture and rapid propagation of Blackberry were studied.The results indicated that the appropriate media for respective culture stages were:for germination of axillary buds:MS BA1.0mg/L IAA0.1 mg/L;for multiplication:MS BA 1.0 mg/L NAA0.05~0.2mg/L,the multiple for multiplication reached to 8;for rooting:1/2MS IBA0.1mg/L or 1/2MS NAA0.05mg/L and the rate of rooting was 96.3%.Transplanting rooted plantlet to a different substrata,the results showed that substrata which consists of garden-soil,perlite and wormcast was the most suitable for transplant and late growth,and survival rate was up to 96.9%,and transplant plantlet grow healthy.