小麦及其近缘种叶枯病的病斑型及其细胞超微结构特点

应用离体叶片点滴接种平脐蠕孢菌Bipolaris sorokiniana孢子悬浮液并进行电镜观察,研究小麦及其近缘种属叶片上病斑型的发生特点及病、健组织细胞内部超微结构的变化。结果表明,不同品种小麦相同发育阶段的离体叶上病斑型的变化仅与其自身抗病性遗传表达有关。小麦病斑的反应型变化过程,随时间在品种、叶位、不同发育阶段间存在差异,叶肉病变细胞中细胞器结构及功能完好的为14.3%,变化的为28.6%,解体和功能丧失的为57.1%;小麦近缘种病斑的反应型不随时间改变,仅在种属间存在差异,且叶位间的差异不显著,大赖草病变细胞中细胞器结构及功能完好、改变和丧失的均为33.3%。

Lesion types and cell ultrastructure on excised leaf caused by Bipolaris sorokiniana in wheat and its related species

Attempted with lesion types to assess the quantitative resistances of wheat to HLB and utilizing drip-inoculating of conidiospores(Bipolaris sorokiniana) suspension solution on the excised leaf in petri dish and using electron microscopy,we examined the changing of lesion types on the detached leaf segment of wheat and its related species and cellular ultrastructure in both resistant and susceptible genera.The results indicated that the lesion types on wheat leaf at same growth stage caused by the fungi were related only to their own genetic resistance of the wheat genotypes.Two kinds of lesion types in the test materials were found:wheat reaction types of the lesion were altering with time,differed in leaf arrangement and varieties,with the cellular structure and function at 14.3% intact,28.6% changed,57.1% lost in the diseased cells,respectively;wheat related species,which were not altering with time,existed variation only in species or genera as well as a few in leaf arrangement,with the same percentage of 33.3% for cellular structure and function intact,changed and lost.Organelles altered in the diseased cells both for wheat and its related species were observed.