5-氮杂胞苷调节植物基因表达研究进展与应用展望

5-氮杂胞苷是一种DNA甲基化抑制剂,可以通过降低DNA的甲基化水平来调节基因的表达,从而对生物的生长发育进行表观遗传调控,其在动物与医学领域已有广泛的研究与应用,近年来在植物基因表达调节方面的研究与应用报道也逐渐增多。本研究对植物DNA甲基化的类型、引发、影响因素和抑制剂的作用进行了概述,重点对5-氮杂胞苷对植物基因表达水平的调节和作用效果进行了阐述。并对其在水稻、小麦、棉花等重要作物生长发育、逆境适应、育性调节及次生代谢的影响与作用进行归纳,可为植物基因表达表观调节研究与应用提出新的关注点。

5-Azacytidine Regulating Plant Gene Expression: Research Progress and Application Prospect

5-Azacytidine is a DNA methylation inhibitor, which can regulate gene expression by reducing the methylation level of DNA, thus providing epigenetic regulation for biological growth and development. It has been widely studied as well as applied in animal and medical field and its research and application in plant gene expression regulation have been reported increasingly in recent years. This review provided an overview of the types of plant DNA methylation, the causing factors, the influencing factors and the role of inhibitors, focusing on the regulation and effects of 5-Azacytidine on plant gene expression levels. The effects of 5-Azacytidine on growth and development, stress adaptation, fertility regulation and secondary metabolism in rice, wheat, cotton and other important crops were summarized, and a new insight for the study and application of epigenetic regulation of plant gene expression was also presented.