| 鹿花盘蛋白质的提取与生物活性测定 |
| |
| 引用本文： | 苏凤艳,李慧萍,王艳梅,黄玉霞,肖寿明,肖宇奇.鹿花盘蛋白质的提取与生物活性测定[J].猪业科学,2001,18(2):18-20. |
| |
| 作者姓名： | 苏凤艳 李慧萍 王艳梅 黄玉霞 肖寿明 肖宇奇 |
| |
| 作者单位： | 1. 吉林农业大学中药材学院,
2. 吉林省中医中药研究所 |
| |
| 摘 要： | 采用盐提、热变性处理及凝胶过滤法从鹿花盘中提取 鹿花盘蛋白质(PSAB)。PSAB经SDS-PAGE电泳呈现5条谱带，测定其分子量分别为65.57KD、2 8.179KD、21.265KD、16.758KD、13.79KD；氨基酸分析表明，鹿花盘蛋白质含有17种氨基酸 。动物实验表明，鹿花盘蛋白质能显著地增强机体的抗疲劳作用和提高肾上腺功能；对油漆 应激小鼠有明显保护作用，能显著增加小鼠红细胞数和血红蛋白的含量。表明PSAB具有滋补 强壮、养气补血的作用，是鹿花盘的活性成分之一。
|
| 关 键 词： | 鹿花盘 提取分离 活性成分 生物效应 |
| 文章编号： | 008－9381（2001）02－0018－03 |
| 修稿时间： | 2000年11月26 |
Protein Component Extractio n and its Bioactivity Determination of Sika Deer Antler Base |
| |
| Su Fengyan,LI Huiping,WANG Yanmei,Huang Yuxia,Xiao Souming,Xiao Yuqi.Protein Component Extractio n and its Bioactivity Determination of Sika Deer Antler Base[J].Swine Industry Science,2001,18(2):18-20. |
| |
| Authors: | Su Fengyan LI Huiping WANG Yanmei Huang Yuxia Xiao Souming Xiao Yuqi |
| |
| Abstract: | Protein component of sika deer antler base（PSAB） was extracted by means of salty extraction，boiling and g el filteration．Five belts were determine d by SDS-PAGE，and the molecular weight o f them were 65．57 KD，28．179 KD，21．265 KD ，16．785 KD and 13．79 KD respectively．The re were seventeen kinds of amino acid in PSAB by Model 835～50 High Speed Amino A cid Analyzer．
The results showed that PSAB can protr act the swimming time（P＜0．00 1） and strengthen the function of adrena l gland and hematopoiesis(P＜ 0．05)．PSAB also stimulated the stress re sistence white rats in painting（P WTBZ〗＜0．05），and can raise the number of red cell，the content of hemoglobin of wh ite rats(P＜0．05)．
The results showed that PSAB possessed the functions of nourishing and strengt hening body，developing immunity from dis ease，resisting inflammation and enrichin g blood．It is one of the principal activ e component of the Sika deer antler base． |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
|
