| 进境大丽花种子携带大丽花花叶病毒的分子检测 |
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| 引用本文： | 陈青,黄峰,廖富荣,林石明,陈红运.进境大丽花种子携带大丽花花叶病毒的分子检测[J].植物检疫,2012(2):20-22. |
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| 作者姓名： | 陈青 黄峰 廖富荣 林石明 陈红运 |
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| 作者单位： | 厦门出入境检验检疫局 |
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| 摘 要： | 通过生长性试验、症状观察、PCR扩增及序列测定,从一批进境的大丽花种子上发现大丽花花叶病毒(Dahlia mosaic virus,DMV)。序列比对结果表明,发现的DMV分离物与DMV-D10存在较近的亲缘关系。根据已报道引物P3/P4的测序结果,并将其与已有的DMV序列进行比对,设计1对预期扩增片段为750 bp的检测引物P5/P6。实验结果证实,P5/P6的扩增效率优于已报道的引物P1/P2和P3/P4,适用于DMV的分子检测。
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| 关 键 词： | 大丽花花叶病毒 分子检测 |
Molecular detection of seed transimitted Dahlia mosaic virus from an imported Dahlia seed lot |
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| Chen Qing,Huang Feng,Liao Furong,Lin Shiming,Chen Hongyun.Molecular detection of seed transimitted Dahlia mosaic virus from an imported Dahlia seed lot[J].Plant Quarantine,2012(2):20-22. |
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| Authors: | Chen Qing Huang Feng Liao Furong Lin Shiming Chen Hongyun |
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| Institution: | (Xiamen Entry-Exit Inspection and Quarantine Bureau,Xiamen 361026,China) |
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| Abstract: | Dahlia mosaic virus(DMV) was identified through growing out,symptomatology,PCR amplification and sequence determination from an imported dahlia seed lot.Sequence comparison showed that the identified DMV isolate was closely related to DMV-D10.A primer pair P5/P6 with expected fragment of 750 bp was designed based on the determined sequence and alignment with other DMV isolates.The PCR assay results proved that the primer pair P5/P6 could amplify DMV efficiently compared to previously reported primers,which indicated P5/P6 was suitable for the detection of DMV. |
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| Keywords: | Dahlia mosaic virus molecular detection |
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