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The evaluation of a new rapid milk progesterone test as an aid to improving dairy herd fertility
Authors:A I Worsfold  J M Booth  P W Wells  A C Huddart  C J Stanley
Affiliation:1. Animal Health Division, Hoechst UK Ltd., Walton Manor, Walton, Milton Keynes, Bucks., MK7 7AJ, U.K.;2. Prospect House, Church Brow, Clitheroe, Lancs., U.K.;3. IQ (Bio) Ltd., Downham House, Downhams Lane, Milton Road, Cambridge CB4 1XG U.K.;1. School of Pharmacy, North Sichuan Medical College, Nanchong 637100, China;2. Department of Stomatology, North Sichuan Medical College and Affiliated Hospital of North Sichuan Medical College, Nanchong 637100, China;3. Innovative Platform of Basic Medical Sciences, Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, Nanchong 637100, China;1. Laboratory of Reproductive Technologies and Innovations in Animal Models, Federal Rural University of Semi-Arid, Mossoró, RN, Brazil;2. Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid, Mossoró, RN, Brazil;3. Laboratory of Anatomy, Federal Rural University of the Semi-Arid, Mossoró, Brazil;1. Foundation for Research and Technology Hellas (FORTH), Institute of Electronic Structure and Laser (IESL), N Plastira 100, 70013 Heraklion, Greece;2. Kroto Research Institute, Department of Materials Science and Engineering, University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK;3. Department of Materials Science and Technology, University of Crete, PO Box 2208, 71303 Heraklion, Greece;1. Institute of Dentistry, University of Turku, Turku, Finland;2. Faculty of Dentistry, University of Hacettepe, Ankara, Turkey;3. Oral Health Care, Welfare Division, City of Turku, Turku, Finland;4. Faculty of Dentistry, University of Gazi, Ankara, Turkey
Abstract:A new enzyme-amplified immunoassay (AELIAtm) for the measurement of progesterone in bovine milk has been developed which offers advantages over the radioimmunoassay (RIA) methods currently in use. The benefits of this new system include the use of monoclonal antibodies and an enzyme-amplification system which results in a rapid yet highly sensitive test that is ideally suited for use in veterinary laboratories. This paper describes a trial using this new system for the confirmation of oestrus and for the determination of pregnancy 24 days after insemination. Accuracy of interpretation was slightly improved using the AELIAtm technique which suggests that this new method provides a suitable alternative to the RIA systems currently in use. The speed and sensitivity achieved using the AELIAtm provides great potential for its use as an integral part of a herd fertility scheme.
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