黄色马蹄莲组培快繁体系研究

目的]研究黄色马蹄莲的组培快繁体系。方法]以黄色马蹄莲(Zantedeschia elliotiana Engler)块茎为外植体,系统研究黄色马蹄莲离体再生体系。结果]块茎最佳消毒方法为0.1%升汞处理20 min后接入附加抗生素的培养基培养,无菌率高达93.33%;侧芽萌发与愈伤组织诱导的最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+蔗糖30 g/L,侧芽萌发的数量多且产生的愈伤组织质量好;最佳继代培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+蔗糖30 g/L,增殖系数达4.73,且产生的芽苗生长健壮;最佳生根培养基为1/2MS+IBA 0.4 mg/L+蔗糖20 g/L,根系生长快而健壮,生根率达100%;最佳移栽基质为腐质土∶椰糠∶珍珠岩=1∶1∶2(V/V/V),移栽成活率达98%,移栽苗长势良好。结论]该研究为黄色马蹄莲组培快繁最佳培养条件和培养方法的确定提供了理论和试验依据。

Study on Tissue Culture and Rapid Propagation System of Zantedeschia elliotiana Engler

Objective ] The paper was to study the tissue culture and rapid propagation system of Zantedeschia elliotiana Engler. Method ] U- sing tubers of Z. elliotiana Engler as explants, the regeneration system in vitro of Z. elliotiana Engler was studied. Result ] The best method of disinfection for tubers was soaked in O. 1% HgC12 and moved into the medium with antibiotic after 20 min, then the sterile rate was as high as 93. 33% ; the best medium of lateral bud germination and callus induction was MS ＋6-BA 2.0 mg,/L ＋ NAA 0.1 mg/L ＋ sucrose 30 g/L with high number of lateral bud and good quality of callus ; the best subculture medium was MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.2 mg/L ＋ sucrose 30 g/L with the proliferation coefficient of 4.73, and the bud seedlings grew robust ; the best rooting medium was 1/2MS ＋ IBA 0.4 mg/L ＋ sucrose 20 g/L with the rooting rate of 100%, and the root grew fast and robust; the best transplanting matrix was humus soil： coconut husk： perlite = l： 1： 2（ V~ V/V） with the transplanting survival rate of 98%, and the transplanting seedlings grew well. Conclusion] The study provided theoretical and experimental basis for the determination of the best culture conditions and culture methods of tissue culture and rapid propagation of Z. elliotiana