甜菜-甜菜坏死黄脉病毒互作过程中过氧化氢的积累与分布

以甜菜抗、感丛根病品种与甜菜坏死黄脉病毒互作体系为研究对象，检测不同体系间H₂O₂的产生和积累，并利用电镜细胞化学标记技术在亚细胞水平上对H₂O₂的空间分布定位,探讨H₂O₂积累和分布与甜菜抗丛根病性的关系。结果表明, 甜菜抗、感丛根病体系均出现大量H₂O₂，其中抗病体系的H₂O₂产生量明显高于感病体系。H₂O₂在抗病和感病体系中的分布位置基本相似，多分布在块根、叶脉细胞的液泡膜和质膜上，叶脉细胞间隙也有H₂O₂的分布，但不同体系间H₂O₂含量有着明显差异,感病体系H₂O₂沉积量明显弱于抗病体系。研究结果说明，H₂O₂产生量和分布与甜菜抗丛根病性有密切联系，H₂O₂参与了甜菜对病毒侵染的防御反应。

Accumulation and Distribution of Hydrogen Peroxide in Interaction between Sugarbeet Plant and Sugarbeet Necrotic Yellow Vein Virus

Hydrogen peroxide（H₂O₂）plays an important role in the interaction between plant and pathogen defense response.The objective of this research was owing the interaction system of beet necrotic yellow vein virus（BNYVV）and resistant,susceptible sugarbeet lines to disclose the relationship of accumulation and distribution of H₂O₂ with sugarbeet rhizomania resistance.The production and accumulation of H₂O₂ in the two interaction systems were detected and the distribution of H₂O₂ was observed in subcellular level using the cytochemical technique.The result showed that there was plenty of H₂O₂ in both interaction systems,and the production of H₂O₂ was evidently higher in the resistant lines than with in the susceptible lines.In addition,distribution and the location of H₂O₂ were similar in the two lines,where we observed mainly in the tonoplast and the plasma membrane of host root,vein cells as well as the intercellular space of some host vein cells.But there were obvious differences in the content of H₂O₂ accumulated in the two interaction lines,the deposition of H₂O₂ in the susceptible line was significantly lower than that in the resistant line,showing it may have a close relationship with resistance of sugarbeet against rhizomania,H₂O₂ showed the involved in the defense responses to virus infection in sugar beets.