| 基于抑制消减杂交开发长穗偃麦草(Lophopyrum elongatum)特异分子标记 |
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| 引用本文： | 葛江燕,陈士强,高营营,高勇,朱雪,黄泽峰,陈建民.基于抑制消减杂交开发长穗偃麦草(Lophopyrum elongatum)特异分子标记[J].作物学报,2012,38(10):1818-1826. |
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| 作者姓名： | 葛江燕 陈士强 高营营 高勇 朱雪 黄泽峰 陈建民 |
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| 作者单位： | 扬州大学生物科学与技术学院，江苏扬州 225009 |
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| 基金项目： | 国家自然科学基金项目(31071406);江苏省普通高校研究生科研创新计划项目(2011-997)资助 |
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| 摘 要： | 分别以二倍体长穗偃麦草和普通小麦中国春的基因组DNA作实验组和对照组，运用抑制消减杂交(SSH)技术去除2个基因组DNA之间的同源序列, 获得长穗偃麦草特异的DNA片段构建的单向抑制消减文库，并对随机克隆测序。根据测序结果设计引物，获得36个长穗偃麦草基因组特异分子标记，成功率高达69.2%。这些标记均能在具有长穗偃麦草E染色体的不同小麦背景和不同世代中稳定表现，可用于小麦背景中跟踪长穗偃麦草染色体或片段。
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| 关 键 词： | 普通小麦 长穗偃麦草 基因组特异分子标记 SSH |
| 收稿时间： | 2012-02-23 |
Development of Genome-Specific Molecular Markers for Lophopyrum elongatum Based on Suppression Subtractive Hybridization |
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| GE Jiang-Yan, CHEN Shi-Qiang, GAO Ying-Ying, GAO Yong, ZHU Xue, HUANG Ze-Feng, and CHEN Jian-Min.Development of Genome-Specific Molecular Markers for Lophopyrum elongatum Based on Suppression Subtractive Hybridization[J].Acta Agronomica Sinica,2012,38(10):1818-1826. |
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| Authors: | GE Jiang-Yan CHEN Shi-Qiang GAO Ying-Ying GAO Yong ZHU Xue HUANG Ze-Feng and CHEN Jian-Min |
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| Institution: | College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China |
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| Abstract: | The unidirectional subtracted library of the tester Lophopyrum elongatum (2n=2x=14, EE) and the driver wheat (Triticum aestivum cv. Chinese Spring, 2n=6x=42, AABBDD) was constructed by suppression subtractive hybridization (SSH) method to remove the homologous sequences and enrich the difference of sequences between L. elongatum and Chinese Spring. After cloning and sequencing the specific fragments of L. elongatum, we obtained 36 genome-specific molecular markers of L. elongatum with the efficiency of 69.2%. These markers could be stably inherited in different wheat backgrounds and different hybrid generations with L. elongatum chromosomes, indicating that they are applicable in the detection of chromosomes or chromosomal fragments of L. elongatum in wheat background. |
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| Keywords: | Triticum aestivum Lophopyrum elongatum Genome-specific molecular marker Suppression subtractive hybridization |
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