| 小麦抗秆锈病基因Sr33的微卫星标记 |
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| 引用本文： | 韩建东,李伟华,曹远银,宫志远,姚强.小麦抗秆锈病基因Sr33的微卫星标记[J].作物学报,2012,38(6):1003-1008. |
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| 作者姓名： | 韩建东 李伟华 曹远银 宫志远 姚强 |
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| 作者单位： | 1.山东省农业科学院农业资源与环境研究所，山东济南 250100；2沈阳农业大学植物免疫研究所，辽宁沈阳 110866 |
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| 基金项目： | 国家公益性行业(农业)科研专项(200903035);国家自然科学基金项目(31171829);国家“十二五”科技支撑计划项目(2012BAD19B04)资助 |
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| 摘 要： | 小麦抗秆锈病基因Sr33对强毒力小种Ug99和我国多数小麦秆锈菌小种具有良好抗性。以感病品种McNair701和来源于Tetra Canthatch/Triticum tauschii的单基因系Sr33为亲本，选用我国流行的小种34MKG，对作图群体161个F2单株及其F2:3家系进行抗性鉴定分析，利用分离群体集群分析法对位于小麦1D染色体上的57对微卫星引物进行扩增多态性筛选。获得2对在亲本及F2抗、感群体间揭示多态性的共显性标记 Xbarc152和Xcfd15，位于Sr33两侧，与目标基因的遗传距离分别为2.4 cM和2.1 cM。
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| 关 键 词： | 微卫星标记 小麦秆锈病 Sr33 基因定位 |
| 收稿时间： | 2011-09-04 |
Microsatellite Markers Linked to Stem Rust Resistance Gene Sr33 in Wheat |
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| HAN Jian-Dong
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LI Wei-Hua
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CAO Yuan-Yin
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GONG Zhi-Yuan
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YAO Qiang.Microsatellite Markers Linked to Stem Rust Resistance Gene Sr33 in Wheat[J].Acta Agronomica Sinica,2012,38(6):1003-1008. |
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| Authors: | HAN Jian-Dong LI Wei-Hua CAO Yuan-Yin GONG Zhi-Yuan YAO Qiang |
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| Institution: | 1.Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, Jinan 250100, China;2.Institute of Plant Immunology, Shenyang Agricultural University, Shenyang 110866, China |
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| Abstract: | Gene Sr33 confers resistance not only to Ug99,but also to most Puccinia graminis f.sp.tritici races in China.A mapping population,consisting of 161 F2 plants and F2:3 families derived from the cross between susceptible wheat cultivar "McNair701" and a Tetra Canthatch/Triticum tauschii line carrying single Sr gene Sr33,were inoculated with Chinese prevalent race 34MKG to locate this resistance gene using microsatellite markers.A total of 57 microsatellite markers covering chromosome 1D were used in bulked segregation analysis(BSA) based on resistance identification at seedlings stage.Two codominant markers,Xbarc152 and Xcfd15,were obtained,which were located at both sides of Sr33.The genetic distances of Xbarc152 and Xcfd15 to the target gene were 2.4 cM and 2.1 cM,respectively.These closely linked markers can be used in marker-assisted selection of wheat breeding against stem rust disease. |
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| Keywords: | Microsatellite marker Wheat stem rust Sr33 Gene location |
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