| 禽流感病毒H9N2亚型分离株HA基因的克隆及序列分析 |
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| 引用本文： | 唐志芬,贾永清,王君伟,孙进华,管雪婷,高宏丽.禽流感病毒H9N2亚型分离株HA基因的克隆及序列分析[J].畜牧与兽医,2004,36(12):3-5. |
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| 作者姓名： | 唐志芬 贾永清 王君伟 孙进华 管雪婷 高宏丽 |
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| 作者单位： | 东北农业大学动物医学院,黑龙江,哈尔滨,150030 |
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| 摘 要： | 以禽流感病毒地方株A/chicken/Mudanjiang/ 0 82 3/ 2 0 0 0 (H9N2 )的总RNA为模板 ,用通用反转录引物和自行设计的一对特异性引物 ,采用RT PCR方法扩增了HA基因。测序及序列分析结果表明 :HA基因全长 1 70 7bp ,编码 560个氨基酸残基 ,与GenBank收录的H9N2亚型的核苷酸同源性最高达 99% ,最低为 87 2 3 % ;氨基酸同源性最大达 98 0 4 % ,最低为 88 57%。经HA裂解位点附近的氨基酸序列分析表明 ,CMJ/ 0 0毒株为低致病力毒株
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| 关 键 词： | 禽流感病毒 HA基因 克隆 序列分析 |
| 文章编号： | 0529-5130(2004)12-0003-03 |
| 修稿时间： | 2004年3月31日 |
Cloning and sequence analysis of HA gene of H9N2 isolate of Avian influenza viruses |
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| TANG Zhi-fen,JIA Yong-qing,WANG Jun-wei,SUN Jin-hua,GUAN Xue-ting,GAO Hong-li.Cloning and sequence analysis of HA gene of H9N2 isolate of Avian influenza viruses[J].Animal Husbandry & Veterinary Medicine,2004,36(12):3-5. |
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| Authors: | TANG Zhi-fen JIA Yong-qing WANG Jun-wei SUN Jin-hua GUAN Xue-ting GAO Hong-li |
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| Institution: | TANG Zhi-fen,JIA Yong-qing,WANG Jun-wei~*,SUN Jin-hua,GUAN Xue-ting,GAO Hong-li |
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| Abstract: | The total RNA extracted from local isolated H9N2 avian influenza virus (A/chicken/Mu dan jiang/0823/2000) was used as the template in this study, a piece of universal primer and a pair of specific primer were designed for RT-PCR to amplify the HA gene. A specific 1707 bp cDNA product was obtained, which encodes 560 amino acid residues, and the uncleotide homology with H9N2 subtype publicized in Genbank was between 99% and 87.23%. Furthermore, the homology was between 98.04% and 88.57% at amino acid level, and the amino acid sequence of HA cleavage site indicated that CMJ/00 strain had low virulence. |
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| Keywords: | Avian influenza virus HA gene clone sequence analysis |
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