| 源自动物粪便苏云金芽胞杆菌的cry基因型鉴定 |
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| 引用本文： | 吴昌标. 源自动物粪便苏云金芽胞杆菌的cry基因型鉴定[J]. 福建农林大学学报(自然科学版), 2010, 39(1) |
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| 作者姓名： | 吴昌标 |
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| 作者单位： | 福建农林大学教育部生物农药与化学生物学重点实验室,福建,福州,350002;福建农业职业技术学院,福建,福州,350119 |
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| 基金项目： | 福建省科技厅重点项目(2008N0050);;福建省自然科学基金资助项目(2009J01100) |
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| 摘 要： | 从5种草食性哺乳动物的粪便中筛选获得8株苏云金芽胞杆菌(Bt);并用聚合酶链式反应—限制性酶切片段长度多态性(PCR-RFLP)鉴定体系鉴定各菌株的cry基因型.结果表明:BRC-WCB1、BRC-WCB2、BRC-WCB3、BRC-WCB8同时含有cry1和cry2基因;BRC-WCB6仅含有cry1I基因;BRC-WCB5含有cry3基因,但未出现预知的酶切条带;而BRC-WCB4无任何PCR扩增产物.
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| 关 键 词： | 苏云金芽胞杆菌 动物粪便 基因鉴定 |
Identification of cry-type genes of Bacillus thuringiensis isolated from animal feces |
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| WU Chang-biao. Identification of cry-type genes of Bacillus thuringiensis isolated from animal feces[J]. Journal of Fujian Agricultural and Forestry University, 2010, 39(1) |
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| Authors: | WU Chang-biao |
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| Affiliation: | 1.Key Laboratory of Ministry of Education for Bio-pesticide and Chemistry Biology;Fujian Agriculture and Forestry University;Fuzhou;Fujian 350002;China;2.Fujian Vocational College of Agriculture;Fujian 350119;China |
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| Abstract: | Eight Bacillus thuringiensis(Bt) strains were isolated from five kinds of herbivorous animal feces,and the cry-type genes were identified by using the identification system of polymerase chain reaction-restriction fragments length polymorphism(PCR-RFLP).It indicated that four Bt strains(BRC-WCB1,BRC-WCB2,BRC-WCB3,and BRC-WCB8) contained both cry1 and cry2 genes,BRC-WCB6 contained cry1I gene only,BRC-WCB5 contained cry3 gene without any known digested fragment,while BRC-WCB4 showed no any PCR product. |
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| Keywords: | Bacillus thuringiensis animal feces gene identification |
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