| 基因芯片技术在植物基因克隆中的应用研究进展 |
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| 引用本文： | 孙兵,闫彩霞,张廷婷,郑奕雄,毕玉平,陈高,单世华.基因芯片技术在植物基因克隆中的应用研究进展[J].广西农业生物科学,2009(1):153-158. |
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| 作者姓名： | 孙兵 闫彩霞 张廷婷 郑奕雄 毕玉平 陈高 单世华 |
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| 作者单位： | 东北农业大学;山东省花生研究所;山东农科院高新技术研究中心;仲恺农业工程学院; |
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| 基金项目： | 国家自然基金项目(30771361);;山东省农科院创新基金项目(2006YCX012)资助 |
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| 摘 要： | 基因芯片是以预先设计的方式将大量的生物讯息密码（寡核苷酸、cDNA、基因组DNA等）固定在玻片、硅片等固相载体上组成的密集分子阵列。基因芯片技术本质是生物信号的平行分析，它利用核酸分子杂交原理，通过荧光标记技术检测杂交亲和与否，再经过计算机分析处理可迅速获得所需信息。由于其具有高通量、微型化、连续化、自动化、快速和准确等特点，已引起国际国内广泛的关注和重视，在许多领域得到了广泛的应用。本文简述了基因芯片的概念，技术特点及主要分类，着重对其在基因表达水平检测，基因突变和多态性的分析，基因组DNA分析，后基因组学研究以及转基因农作物检测等方面进行阐述，并说明其存在的问题及展望。
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| 关 键 词： | 基因芯片 基因克隆 植物 |
Research Progress of Gene Chip Technology in Plant Gene Cloning |
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| Sun Bing , Yan Caixia Zhang Tingting Zheng Yixiong Bi Yuping Chen Gao , Shan Shihua Northeast Agricultural University,Harbin, Sh,ong Peanut Research Institute,Qingdao, Zhongkai University of Agriculture , Engineering,Guangzhou, High-Tech Research Center,Sh,ong Academy of Agricultural Sciences,Ji'nan.Research Progress of Gene Chip Technology in Plant Gene Cloning[J].Journal of Guangxi Agricultural and Biological Science,2009(1):153-158. |
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| Authors: | Sun Bing Yan Caixia Zhang Tingting Zheng Yixiong Bi Yuping Chen Gao Shan Shihua Northeast Agricultural University Harbin Sh ong Peanut Research Institute Qingdao Zhongkai University of Agriculture Engineering Guangzhou High-Tech Research Center Sh ong Academy of Agricultural Sciences Ji'nan |
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| Institution: | Sun Bing 1,2 Yan Caixia 2 Zhang Tingting 2 Zheng Yixiong 4 Bi Yuping 3 Chen Gao 1,2 Shan Shihua 2 1 Northeast Agricultural University,Harbin,150030,2 Sh,ong Peanut Research Institute,Qingdao,266100,3 Zhongkai University of Agriculture , Engineering,Guangzhou,510225,4 High-Tech Research Center,Sh,ong Academy of Agricultural Sciences,Ji'nan,250100 |
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| Abstract: | Gene chips are intensive molecular array that lots of biological message password take root with glass,silicon,such as solid-phase carrier by pre-designed manner.The parallel analysis of biological signals is the nature of gene chip technology,it detects affinity or not of hybridize by fluorescent labeling with principle of nucleic acid hybridization,it can obtain the necessary information quickly by computer analysis.Due to the characteristic of high-throughput,miniaturization,continuous,automated,rapid an... |
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| Keywords: | Gene chips Gene cloning Plant |
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