筇竹QtKAT1基因的克隆与表达分析

为探究竹类植物K+调控机理及K+通道的系统,本研究以筇竹(Qiongzhuea tumidinoda)为试验材料,对筇竹QtKAT1基因分离克隆及表达分析.通过克隆获得筇竹QtKAT1基因,全长为3 124bp,含2 235 bp的最大开放阅读框,编码744个氨基酸.生物信息学分析表明QtKAT1基因预测编码蛋白分子质...

Cloning and Expression Analysis of QtKAT1 Gene in Qiongzhuea tumidinoda

In order to explore the K+regulation mechanism and K+channel system of bamboo plants,the QtKAT1 gene was cloned and its expression pattern was analyzed from Qiongzhuea tumidinoda.The full length of QtKAT1 gene was 3124 bp,containing a largest open reading frame of 2235 bp,encoding 744 amino acids.Bioinformatics analysis showed that the predicted molecular weight of the protein was 85.4 kD,containing 6 transmembrane domains.The theoretical isoelectric point number was 6.90,the instability coefficient of the protein was 39.53,the hydrophobic coefficient was 75.81,and average hydrophilicity was-0.261,which was hydrophilic.Phylogenetic tree was constructed using the amino acid sequence encoded by QtKAT1 gene,we can distinguish different families of plants.It is speculated that QtKAT1 gene can be used as one of the molecular markers of plant system classification.The results of real-time fluorescence quantitative analysis showed that the expression of QtKAT1 gene in different tissue parts of Qiongzhuea bambusa seedlings was leaf>stem>root,and the expression in leaf was about 25 times that of root,which illustraing that the expression level of QtKAT1 gene was tissue specific.