基于相对迁移率的节节麦高分子量谷蛋白亚基组成分析

高分子量谷蛋白亚基(HMW-GS)是影响小麦烘烤品质的重要因素。新的HMW-GS变异类型的发现和利用,有助于优质小麦品种的培育。然而,目前HMW-GS新变异类型众多、对照标准材料多样,导致传统数字命名系统分辨率严重降低。本研究通过利用HMW-GS的条带与中国春1Dx2条带的迁移率比值作为相对迁移率来表示亚基类型,进而提高HMW-GS的分辨率,并且不需要除中国春以外的其它对照材料。在158份节节麦中,成功检测到了64种亚基类型和127种亚基组合。对这些亚基组合进行聚类分析,结果显示在遗传相似系数(GS)为0.003的水平上可将其划分为9个类群。上述结果表明,本研究采用的基于相对迁移率的亚基表示方法,能有效区分SDS-PAGE胶上距离较近的蛋白条带,可为麦类作物HMW-GS变异类型的发掘提供新的手段。

Composition Analysis of High Molecular Weight Glutenin Subunits in Aegilops tauschii Cosson Based on Relative Mobility Ratio

High molecular weight glutenin subunit(HMW-GS)is an important factor affecting the baking quality of wheat.The discovery and use of new variants of HMW-GS conduce to cultivate breed new high quality novel wheat varieties with high quality.However,the large number of new variants of HMW-GS and the diversity of standard reference materials have led to a serious reduction in the resolution of traditional digital naming systems.In this study,the position of each protein band was determined by using the ratio of the migration to the Chinese Spring 1 Dx2 band as the relative migration rate,so as to improve the resolution of HMW-GS,and other control materials other than Chinese Spring were not required.A total of 64 subunit types and 127 subunit compositions were detected in 158 A.tauschii.Cluster analysis of these subunit combinations showed that they could be classified into 9 groups at the level of genetic similarity coefficient(GS)of 0.003.The above results indicated that the relative migration rate used in this study could effectively distinguish the relatively close protein bands on SDSPAGE gel,and did not require reference materials other than Chinese Spring,which could provide a new method for the discovery of novel variants of HMW-GS in wheat crop.