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| 杉木ClSAUR25基因5'侧翼序列的克隆与生物信息学分析 | |
| 引用本文： | 饶丽莎,李茂,戴明金,叶义全,曹光球,林思祖,许珊珊. 杉木ClSAUR25基因5'侧翼序列的克隆与生物信息学分析[J]. 分子植物育种, 2021, 19(4): 1107-1112 |
| 作者姓名： | 饶丽莎 李茂 戴明金 叶义全 曹光球 林思祖 许珊珊 |
| 作者单位： | 福建农林大学林学院,福州,350002;国家林业和草原局杉木工程技术研究中心,福州,350002;福建省三明市三明二中总校列东中学分校,三明,365000;福建农林大学林学院,福州,350002;国家林业和草原局杉木工程技术研究中心,福州,350002;福建农林大学林学院,福州,350002 |
| 基金项目： | 国家自然科学基金项目(31600506);福建农林大学林学高峰学科(71201800738)共同资助。 |
| 摘 要： | S4UR(Small auxin-up RNA)基因作为生长素信号早期响应基因,在调控植物生长发育以及对逆境胁迫的应答中扮演着关键角色.本研究通过染色体步移法从杉木基因组DNA中获得了ClSAUR25基因5'侧翼序列,并对其进行了生物信息学分析.结果表明,克隆获得ClSAUR25基因上游5'侧翼序列1 471 bp,该... |
| 关 键 词： | 杉木 SAUR 调控序列 顺式作用元件 |
Cloning and Bioinformatics Analysis of ClSAUR25 Gene 5'Flanking Sequence in Cunninghamia lanceolata |
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| Rao Lisha,Li Mao,Dai Mingjin,Ye Yiquan,Cao Guangqiu,Lin Sizu,Xu Shanshan. Cloning and Bioinformatics Analysis of ClSAUR25 Gene 5'Flanking Sequence in Cunninghamia lanceolata[J]. Molecular Plant Breeding, 2021, 19(4): 1107-1112 | |
| Authors: | Rao Lisha Li Mao Dai Mingjin Ye Yiquan Cao Guangqiu Lin Sizu Xu Shanshan |
| Affiliation: | (College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,350002;Chinese Fir Engineering Technology Research Center,State Forestry and Grassland Administration,Fuzhou,350002;LiedongMiddle School Branch School,Fujian Sanming No.2Middle School,Sanming,365000) |
| Abstract: | SAUR(Small auxin-up RNA)is the early response genes in auxin signaling and plays a critical role in plant growth and development and plant in response to biotic and abiotic stress.In this study,the 5'flanking sequence of ClSAUR25 was cloned by genome walking and analyzed by bioinformatics software.The results showed that the length of the 5'flanking sequence of ClSAUR25 was 1471 bp with three core promoter regions and five CpG islands.In addition to the abundant quantity of CAAT-box and TATA-box,the 5'flanking sequence of ClSAUR25 also contain cis-elements that response to ethylene and metal,and several cis-regulatory-elements that associated with light,anaerobic,meristematic tissue and endosperm.The results in this study indicated that ClSAUR25 gene promoter may belong to light-inducible promoter and play an important role in Cunninghamia lanceolata under abotic stress and also a vital role in regulating C.lanceolata growth and development. |
| Keywords: | Cunninghamia lanceolata Small auxin-up RNA Control sequence Cis-acting elements |
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