培养条件对奶牛金葡菌5型荚膜多糖产量的影响

[目的]研究不同培养条件对牛源金葡菌5型荚膜多糖产量的影响,以便于CP5的生产制备,从而为开展奶牛金葡菌新型多糖疫苗的研究奠定基础。[方法]从患隐性乳腺炎奶牛乳样中分离金葡菌,鉴定荚膜多糖血清型,对5型荚膜多糖菌株,采用BHI,哥伦比亚固体培养基,mod110三种培养基,每种培养基分别采用固体培养,碳源为葡萄糖的液体培养和碳源为乳糖的液体培养三种方式,共组成9种不同培养条件进行培养,研究培养条件对金葡菌荚膜多糖产量的影响。[结果]不同培养结果表明,与常用哥伦比亚培养基比较,BHI培养基能降低荚膜多糖产量,而mod110培养基能提高荚膜多糖产量;同种培养基,固体培养方式比液体培养方式有更高的荚膜多糖产量,同时乳糖作为碳源可提高荚膜多糖产量。

Effect of Culture Condition on Type 5 Capsular Polysaccharide Production of Stapltylococcus aureus from Diary Cattle

[Objective]The effect of different culture conditions on type 5 capsular polysaccharide production of staphylococcus aureus from diary cattle was studied to provide easy way for CP production and preparation and laid down foundation for carrying out new polysaccharide vaccine research.[Method] Staphylococcus aureus was isolated from milk sample of sick dairy cattle and capsular polysaccharide serotypes were identified.Type 5 capsular polysaccharide was cultured on BHI,solid columbia and mod110 culture media.Glucose and lactose were taken as carbon source for every culture media in solid and liquid state.Therefore 9 different culture conditions were taken to study the effect of culture conditions on capsular polysaccharide production.[Result]Different culture conditions indicated that Comparing with columbia culture media,BHI culture media could decline capsular polysaccharide production and mod110 culture media could increase capsular polysaccharide production.While for same culture media,solid culture media was better for capsular polysaccharide production,meanwhile,taken lactose as carbon source could increase capsular polysaccharide production.