| 向大豆导入几丁质酶基因的初步研究 |
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| 引用本文： | 徐香玲,邹联沛.向大豆导入几丁质酶基因的初步研究[J].大豆科学,1999,18(2):101-107. |
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| 作者姓名： | 徐香玲 邹联沛 |
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| 作者单位： | 哈尔滨师范大学 |
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| 摘 要： | 以根癌农杆菌的Ｔｉ质粒为介导，将抗真菌病的几丁质酶基因，导入默经江省栽培大豆一东农３７号，吉林２８号等１４个品种。从子叶节和下胚轴诱导出丛生芽，并再生植株，经ＰＣＲ和ＤＮＡ分子杂交检测，经ＰＣＲ和ＤＮＡ分子杂交检测的１１３株大豆幼苗中，有７株呈阳性反应，证明几丁质酶基因已导入并整合到大豆的基因组中。
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| 关 键 词： | 几丁质酶基因 转基因植株 大豆 基因 |
A PRELIMINARY STUDY ON TRANSFERRING CHITINASE GENE INTO SOYBEANS |
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| Xu XianglingZou LianpeiLiu WeihuaLi Jilin.A PRELIMINARY STUDY ON TRANSFERRING CHITINASE GENE INTO SOYBEANS[J].Soybean Science,1999,18(2):101-107. |
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| Authors: | Xu XianglingZou LianpeiLiu WeihuaLi Jilin |
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| Institution: | Harbin Normal University 150080 |
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| Abstract: | The chitinase gene was transferred into cultivars Dongnong 37, Jilin 28, et al. with Ti plasmid. Adventitious nuds and regennerated plant were inducted from cotylendonus node and hypocotyl. PCR and DNA dot blotting proved that they were transferred plant.Purified plasmid PBin 437 with chitinase gene was also transferred into several soybeans varidties by the pollen tube technique and 223 seeds were obtained.Among 113 plants germinated from these seeds 7 showed positive reaction in PCR and DNA dot blotting. |
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| Keywords: | Ti plasmid Soybean Chitinase gene Transferred gene plant |
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