| 未成熟与成熟猪囊尾蚴蛋白双向电泳的图谱分析 |
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| 引用本文： | 方文,肖靓靓,包怀恩,牟荣.未成熟与成熟猪囊尾蚴蛋白双向电泳的图谱分析[J].贵州农业科学,2011(9):134-136. |
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| 作者姓名： | 方文 肖靓靓 包怀恩 牟荣 |
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| 作者单位： | 贵阳医学院医学检验系临床生化教研室;贵阳医学院人体寄生虫学教研室; |
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| 基金项目： | 国家自然科学基金项目“三种人体带绦虫入侵中间宿主家猪的分子机制研究”(30660168) |
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| 摘 要： | 为从蛋白质水平揭示猪带绦虫入侵中间宿主家猪的免疫逃避机制,应用双向电泳技术分析未成熟与成熟猪囊尾蚴蛋白质表达差异,进行双向电泳图像软件分析.结果表明:未成熟猪囊尾蚴与成熟猪囊尾蚴蛋白的双向电泳凝胶上分别有(217±13)个、(241±17)个蛋白斑点,未成熟猪囊尾蚴表达上调2倍以上的蛋白有6个,下调2倍以上的蛋白有2个...
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| 关 键 词： | 猪带绦虫 囊尾蚴 双向电泳 蛋白质组 |
Total Protein Analysis of Immature and Mature Cysticerci of Taenia solium by Two-dimensional Electrophoresis |
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| FANG Wen,XIAO Liang-liang,BAO Huai-en,MOU Rong.Total Protein Analysis of Immature and Mature Cysticerci of Taenia solium by Two-dimensional Electrophoresis[J].Guizhou Agricultural Sciences,2011(9):134-136. |
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| Authors: | FANG Wen XIAO Liang-liang BAO Huai-en MOU Rong |
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| Institution: | FANG Wen1,XIAO Liang-liang2,BAO Huai-en2*,MOU Rong2(1.Clinical Biochemistry Office,Department of Medical Test,Guiyang Medical College,Guiyang,Guizhou 550004,2.Department of Parasitology,China) |
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| Abstract: | In order to explore the immune-escape mechanisms of the T.solium cysticercus on the protein level,the differentially expressed proteins of the immature and mature T.solium cysticerci were separated by two-dimensional electrophoresis respectively and analyzed by ImageMaster 2D Plantinum 6.0 software.The results showed that the immature and mature T.solium cysticerci have 217 13 and 241 17 protein spots respectively,the immature T.solium cysticerci have six proteins of more than two times upregulation and two... |
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| Keywords: | Taenia solium cysticercus two-dimensional electrophoresis proteome |
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