| 省沽油茎段初代组织培养的初步研究 |
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| 引用本文： | 苗婷婷,蔡新玲,肖正东,薛正帅,季琳琳,陈雪莲.省沽油茎段初代组织培养的初步研究[J].安徽林业,2011(4). |
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| 作者姓名： | 苗婷婷 蔡新玲 肖正东 薛正帅 季琳琳 陈雪莲 |
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| 作者单位： | 安徽省林业科学研究院;安徽农业大学; |
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| 基金项目： | 安徽省2009年科技攻关计划项目——安徽省省沽油种质资源开发利用基础研究(编号:09010304024) |
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| 摘 要： | 以春季省沽油幼嫩茎梢为试验材料,研究消毒处理、茎段的不同位置、不同植物生长调节剂种类及水平等因素对其腋芽诱导的影响。结果表明:利用0.1%HgCl2对外植体灭菌10min效果最好,茎段中部是省沽油初代培养的最佳部位,腋芽诱导的最适培养基为:MS+6-BA1mg/L+NAA0.2mg/L,最高平均株分化可达2.5以上。
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| 关 键 词： | 省沽油 初代培养 茎段 诱导 |
Preliminary Study on Primary Tissue Culture of Staphylea bumalda |
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| Authors: | MIAO Ting-ting CAI Xin-lin XIAO Zheng-dong XUE Zheng-shuai JI lin-lin CHEN Xue-lian |
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| Institution: | MIAO Ting-ting1,CAI Xin-lin1,XIAO Zheng-dong1,XUE Zheng-shuai2,JI lin-lin1,CHEN Xue-lian1(1.Anhui Academy of Forestry,Hefei 230031,China,2.Anhui Agricultural University) |
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| Abstract: | With the tender stem segments of Staphylea bumalda as the testing materials,the effects of such factors as disinfection treatments,different parts of stem and different types and levels of plant growth regulators on the axillary bud induction were studied.The results showed:The 0.1% HgCl2 10 min had the best sterilization effect;The optimal part in the primary culture of Staphylea bumalda was the middle stem segment;The optimal culture medium for inducing axillary buds was MS+6-BA 1 mg/L +NAA 0.2 mg/L,resul... |
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| Keywords: | Staphylea bumalda Primary culture Stem segments Induction |
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