| 番茄离体培养及快繁殖技术研究 |
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| 引用本文： | 黄刚,孙光英,郑晓峰,李竹莹.番茄离体培养及快繁殖技术研究[J].北方园艺,2011(24):149-151. |
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| 作者姓名： | 黄刚 孙光英 郑晓峰 李竹莹 |
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| 作者单位： | 黔东南民族职业技术学院,贵州 凯里,556000;黔东南民族职业技术学院,贵州 凯里,556000;黔东南民族职业技术学院,贵州 凯里,556000;黔东南民族职业技术学院,贵州 凯里,556000 |
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| 基金项目： | 贵州省黔东南州科技局2007年度州级科技三项费专项经费资助项目(20070023) |
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| 摘 要： | 以番茄种子为试材,采用正交实验方法配制不同的培养基,研究不同浓度的6-BA、NAA、IBA对不定芽诱导及丛生芽增殖的影响.结果表明:愈伤组织诱导的适宜培养基为:MS+6-BA 2.0mg/L+IBA 0.3 mg/L+蔗糖30 g/L;不定芽诱导适宜培养基为:MS+6-BA3.0 mg/L+NAA 0.1 mg/L+蔗糖30 g/L;丛生芽增殖的适宜培养基为:MS+6-BA 1.0mg/L+NAA 0.01 mg/L+蔗糖30 g/L;生根培养基为:1/2 MS+NAA 0.6mg/L+蔗糖30 g/L.
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| 关 键 词： | 番茄 组织培养 快速繁殖 正交实验 |
Study on the Technology Isolated Culture and Rapid Propagation of Tomato |
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| HUANG Gang
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SUN Guang-ying
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ZHENG Xiao-feng
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LI Zhu-ying.Study on the Technology Isolated Culture and Rapid Propagation of Tomato[J].Northern Horticulture,2011(24):149-151. |
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| Authors: | HUANG Gang SUN Guang-ying ZHENG Xiao-feng LI Zhu-ying |
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| Institution: | HUANG Gang,SUN Guang-ying,ZHENG Xiao-feng,LI Zhu-ying(College of Qiandongnan Nation Vocational Technical,Kaili,Guizhou 556000) |
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| Abstract: | Tomato seeds chosen as test material,by the method of orthogonal test of preparation of different media,the effect of different concentrations of 6-BA,NAA,IBA on adventitious bud induction and proliferation of multiple shoot clumps were studied.The results showed that optimum medium for callus induction was MS+6-BA 2.0 mg/L+ IBA 0.3 mg/L+sucrose 30 g/L;optimum medium for adventitious bud induction was MS+6-BA 3.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L;suitable medium for the proliferation buds was MS+6-BA 1.0 mg/L+NAA 0.01 mg/L+sucrose 30 g/L;suitable medium for rooting medium was 1/2MS+NAA 0.6 mg/L+sucrose 30 g/L. |
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| Keywords: | tomato tissue culture rapid propagation orthogonal test |
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