| β-葡聚糖酶降解茯苓多糖的效应 |
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| 引用本文： | 郭雨桐,肖文军.β-葡聚糖酶降解茯苓多糖的效应[J].北方园艺,2011(24):18-22. |
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| 作者姓名： | 郭雨桐 肖文军 |
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| 作者单位： | 湖南农业大学园艺园林学院,湖南长沙410128;国家中医药管理局亚健康干预技术实验室,湖南农业大学,湖南长沙410128;湖南农业大学园艺园林学院,湖南长沙,410128 |
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| 基金项目： | 湖南省科学技术厅资助项目(S2009W2052;2008WK3001) |
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| 摘 要： | 以资源丰富且多糖含量高的茯苓为原料,以茯苓多糖的提取得率为指标,通过酶促降解的单因子试验、正交实验及验证试验,研究了β-葡聚糖酶对茯苓多糖的降解效应,优化筛选了β-葡聚糖酶降解茯苓多糖的技术参数.结果表明:反应温度55℃、时间90 min、pH 5.5、酶浓度6.5％是β-葡聚糖酶降解茯苓多糖的最优技术参数,在该参数条件下,应用β-葡聚糖酶提取茯苓多糖的得率为13.31％,对茯苓多糖的高值化利用具有较好的参考指导价值.
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| 关 键 词： | 茯苓 茯苓多糖 β-葡聚糖酶 酶促降解 提取 |
The Effect of β-Glucanase in Degradation Pachymaran |
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| GUO Yu-tong
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XIAO Wen-jun.The Effect of β-Glucanase in Degradation Pachymaran[J].Northern Horticulture,2011(24):18-22. |
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| Authors: | GUO Yu-tong XIAO Wen-jun |
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| Institution: | GUO Yu-tong1,2,XIAO Wen-jun1(1.College of Horticulture and Landscape,Hunan Agricultural University,Changsha,Hunan 410128;2.State Administration of Traditional Chinese Medicine Sub-health Intervention Technology Laboratory,Hunan Agricultural University,Changsha,Hunan 410128) |
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| Abstract: | With the single-factor test,orthogonal experiment and verification test on enzyme dosage,enzymolysis time,enzymolysis temperature and pH value,using resource-rich,high-polysaccharide Poria cocos as the raw material and extraction rate of pachymaran as the comparative standard,the β-glucanase effective on the degradation of pachymaran systematically and optimized the selection of the β-glucanase’s technical parameters on the degradation of pachymaran were studied.The results showed that when the pH was 5.0,the enzyme dosage was 0.13 g(6.5%),the enzymolysis temperature was 55℃,and the enzymolysis time was 90 min,the extraction rate of pachymaran,using the β-glucanase was 13.31%.It was significantly superior to the traditional yield of pachymaran. |
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| Keywords: | Poria cocos Poria cocos polysaneharide β-glucanase enzymatic degradation extract |
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