| 杉木的组织培养* |
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| 引用本文： | 崔杰,邓素娥,张华,莫志安,连人豪.杉木的组织培养*[J].广东林业科技,2018,34(3):119-122. |
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| 作者姓名： | 崔杰 邓素娥 张华 莫志安 连人豪 |
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| 作者单位： | 广州市石门国家森林公园,广州市石门国家森林公园,广州市石门国家森林公园,广州市石门国家森林公园,广州市石门国家森林公园 |
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| 摘 要： | 以杉木嫩茎为实验材料,建立组织培养体系。结果表明,杉木嫩茎用HgCl2 试剂消毒11min效果最好,污染率和死亡率极低。最佳诱导培养基为MS+6-BA1.5mg/L+NAA0.5mg/L +3%蔗糖。最适宜杉木组培苗生根的培养基为1/2MS+6-BA0.5mg/L+IBA2.0mg/L+2%蔗糖。移栽30d后,成活率达到88%以上。
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| 关 键 词： | 杉木 外植体 组织培养 |
| 收稿时间： | 2017/11/28 0:00:00 |
| 修稿时间： | 2018/1/22 0:00:00 |
Tissue Culture and Micropropagation of Cunninghamia lanceolata |
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| CUI Jie,DENG Sue,ZHANG Hu,MO Zhian and LIAN Renhao.Tissue Culture and Micropropagation of Cunninghamia lanceolata[J].Forestry Science and Technology of Guangdong Province,2018,34(3):119-122. |
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| Authors: | CUI Jie DENG Sue ZHANG Hu MO Zhian and LIAN Renhao |
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| Institution: | Shimen National Forest Park of Guangzhou,Guangzhou,Shimen National Forest Park of Guangzhou,Guangzhou,Shimen National Forest Park of Guangzhou,Guangzhou,Shimen National Forest Park of Guangzhou,Guangzhou,Shimen National Forest Park of Guangzhou,Guangzhou |
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| Abstract: | The tender stem segments of Cunninghamia lanceolata was used for tissue culture and micropropagation. The results showed that 0.1% mercuric chloride solution (11min) was appropriate, the pollution and mortality rate was low. The best medium for proliferation was MS+6-BA1.5mg/L+NAA0.5mg/L+3%S. the most suitable medium for rooting was1/2MS+6-BA0.5mg/L+IBA2.0mg/L +2%S. After 30days, the survival rate was more than 88%. |
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| Keywords: | Cunninghamia lanceolata explants tissue culture |
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