Oxidative damage in bone and erythrocytes of suckling rats exposed to 2,4-dichlorophenoxyacetic acid |
| |
Authors: | Afef TroudiMadiha Sefi Ibtissem Ben Amara Nejla SoudaniAhmed Hakim Khaled Mounir ZeghalTahia Boudawara Najiba Zeghal |
| |
Institution: | a Animal Physiology Laboratory, University of Sfax, UR 11ES70, Faculty of Sciences, BP 1171, 3000 Sfax, Tunisia b Pharmacology Laboratory, University of Sfax, Faculty of Medicine, 3029 Sfax, Tunisia c Histopathology Laboratory, University of Sfax, CHU Habib Bourguiba of Sfax, 3029 Sfax, Tunisia |
| |
Abstract: | Nowadays, people’s exposure to pesticides such as 2,4-dichlorophenoxyacetic acid (2,4-D) is increasing continuously. This compound is suspected to produce in excess free radicals which have adverse effects on human health causing several cell alterations in the organism. The present study investigated oxidative stress in the bone and erythrocytes of suckling rats whose mothers were treated with 2,4-D. Experiments were carried out on adult Wistar rats given 600 mg/L of 2,4-D in their drinking water from the 14th day of pregnancy until day 14 after delivery. Exposing dams to 2,4-D caused disorders in the bone of their progeny. Indeed, it induced changes in bone mineralization, especially calcium and phosphorus levels. Moreover, total tartrate-resistant acid phosphatase, which reflected bone resorption, was enhanced while total alkaline phosphatase, which reflected bone formation, was reduced suggesting that this herbicide accelerated bone resorption. The impairment of bone function corresponded histologically.Rats exposed to 2,4-D showed in both bone and erythrocytes an increase in malondialdehyde, advanced oxidation protein products and protein carbonyl levels and a decrease in non-enzymatic (glutathione, non-protein thiol, and vitamin C) and enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) antioxidant system. |
| |
Keywords: | 2 4-D 2 4-dichlorophenoxyacetic acid ALP total alkaline phosphatase ACP total tartrate-resistant acid phosphatase MDA malondialdehyde AOPP advanced oxidation of protein products PCO protein carbonyls CAT catalase GPx glutathione peroxidase SOD superoxide dismutase GSH glutathione NPSH non-protein thiol AChE acetylcholinesterase BuChE butyrylcholinesterase LPO lipid peroxidation ROS reactive oxygen species |
本文献已被 ScienceDirect 等数据库收录! |
|