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Frequencies of the M918I mutation in the sodium channel of the diamondback moth in China, Thailand and Japan and its association with pyrethroid resistance |
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| Authors: | Shoji Sonoda Xueyan ShiDunlun Song Youjun ZhangJianhong Li Gang WuYong Liu Ming LiPei Liang David WariMasaya Matsumura Chieka MinakuchiToshiharu Tanaka Tadashi MiyataXiwu Gao |
| Affiliation: | a Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan b Department of Entomology, China Agricultural University, Beijing 100193, China c Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China d Department of Plant Protection, Huazhong Agricultural University, Wuhan, Hubei 430070, China e Department of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China f Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, Hunan 410125, China g Crop Protection Institute, Guizhou University, Guiyang, Guizhou 550025, China h National Agricultural Research Center for Kyushu Okinawa Region, Koshi, Kumamoto 861-1192, Japan i Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi 464-8601, Japan |
| Abstract: | In addition to the allele frequencies of the L1014F and T929I mutations which are involved in nerve-insensitive resistance to a pyrethroid, those of the M918I mutation were examined using field strains obtained in China, Thailand, and Japan during 2009-2011. Results show that the resistance allele frequencies at the L1014F site were 89-100%, 97-100% and 65-85%, respectively, for strains in China, Thailand, and Japan. The respective allele frequencies at the T929I site were 86-100%, 70-97% and 58-84% for Chinese, Thai, and Japanese strains. With low frequencies up to 27%, M918I was found in Japan and China, but not in Thailand. The strain homozygous for the M918I and L1014F mutations was established and its resistance level to a pyrethroid was examined. The strain lacks a portion of the sodium channel gene corresponding to the 3′ portion of exon 18a, intron 18, and the 5′ portion of exon 18b. Nevertheless, the strain showed a similar level of resistance to that which was homozygous for the T929I and L1014F mutations. |
| Keywords: | Diamondback moth Insecticide resistance Plutella xylostella Pyrethroid Sodium channel |
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