| 柱状黄杆菌部分生物学特性的研究 |
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| 引用本文： | 黄锦炉,汪开毓,姜婷婷,黄小丽,耿毅,陈德芳. 柱状黄杆菌部分生物学特性的研究[J]. 大连水产学院学报, 2010, 25(6): 506-510 |
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| 作者姓名： | 黄锦炉 汪开毓 姜婷婷 黄小丽 耿毅 陈德芳 |
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| 作者单位： | [1]四川农业大学鱼病研究中心,四川雅安625014 [2]四川农业大学水产系,四川雅安625014 |
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| 基金项目： | 教育部"长江学者和创新团队发展计划"创新团队项目 |
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| 摘 要： | 采用革兰氏染色法、比色法和平板扩散法对柱状黄杆菌Flavobacterium columnare的部分生物学特性进行了研究。结果表明：用肉汤培养柱状黄杆菌24 h时,其形态多样,如半圆形、U型、V型或S型等;培养48 h时,柱状黄杆菌中开始出现颗粒状老龄培养物;培养72 h时,有部分菌体发生的＂分节＂现象,而老龄培养物也增多。用肉汤培养时,该菌的最佳生长条件分别是NaCl浓度为2.0 g/L,pH为7.4,温度为25～28℃。在最佳培养条件下,该菌的胞外产物具有蛋白酶、脂酶和卵磷脂酶活性,然而却检测不到淀粉酶、明胶酶和脲酶活性。
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| 关 键 词： | 柱状黄杆菌 形态学 生长条件 胞外产物 |
The biological characteristics of bacterium Flavobacterium columnare |
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| HUANG Jin-lu,WANG Kai-yu,JIANG Ting-ting,HUANG Xiao-li,GENG Yi,CHEN De-fang. The biological characteristics of bacterium Flavobacterium columnare[J]. Journal of Dalian Fisheries University, 2010, 25(6): 506-510 |
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| Authors: | HUANG Jin-lu WANG Kai-yu JIANG Ting-ting HUANG Xiao-li GENG Yi CHEN De-fang |
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| Affiliation: | 1.Fish Disease Research Center,Sichuan Agricultural University,Ya＇an 625014,China;2.Department of Aquaculture,Sichuan Agricultural University,Ya＇an 625014,China) |
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| Abstract: | The biological characteristics of bacterium Flavobacterium columnare were studied by Gram stain,colorimetry and agar diffusion methods.The bacterium cultured in broth Shieh meddium for 24 hours was showed different shape in the appearance including arch shape,U form,V form or S form and so on.Some of Flavobacterium columnare cultured for 72 hours appeared as branched cells and more granular involution form than that for 48 hours.The extracellular products（ECP） were found to be positive in the protease,lipoidase and lecithinase,but negative in amylase,gelatinase and urease. |
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| Keywords: | Flavobacterium columnar morphology growth condition extracellular product |
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