| 小麦淀粉合酶基因I的克隆及反义和RNAi载体的构建 |
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| 引用本文： | 康国章,刘超,王永华,郭天财,朱云集.小麦淀粉合酶基因I的克隆及反义和RNAi载体的构建[J].西北农业学报,2008,17(2):74-79. |
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| 作者姓名： | 康国章 刘超 王永华 郭天财 朱云集 |
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| 作者单位： | 河南农业大学,国家小麦工程技术研究中心,河南郑州,450002 |
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| 基金项目： | 中国博士后科学基金(20060390773)，河南省教育厅自然科学基金(2006210007) |
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| 摘 要： | 采用RT-PCR方法从小麦品种豫教2号发育的籽粒中克隆出淀粉合酶I基因(starch synthase I,SSI)部分cDNA片段(795 bp)(GenBank No.EF221762),同源性比较结果显示,它与GenBank上已报道的SSI基因有高度同源性。以p WM101质粒为基础,构建了由35S启动子调控的SS I基因的反义表达载体p WM101SSI;另外,还以pFGC5941质粒为基础,构建了SSI基因的RNAi载体pFGC5941SSIsa,这些载体的构建为研究此基因的功能奠定了基础。
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| 关 键 词： | 普通小麦 淀粉合酶基因I 表达载体 |
| 文章编号： | 1004-1389(2008)02-0074-06 |
| 收稿时间： | 2007/10/19 0:00:00 |
| 修稿时间： | 2007年10月19 |
Molecular Cloning of Starch Synthase I Partial Sequences and Construction of Antisense and RNAi Expression Vectors |
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| KANG Guo-zhang,LIU Chao,WANG Yong-hu,GUO Tian-cai and ZHU Yun-ji.Molecular Cloning of Starch Synthase I Partial Sequences and Construction of Antisense and RNAi Expression Vectors[J].Acta Agriculturae Boreali-occidentalis Sinica,2008,17(2):74-79. |
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| Authors: | KANG Guo-zhang LIU Chao WANG Yong-hu GUO Tian-cai and ZHU Yun-ji |
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| Institution: | National Engineering Research Centre for Wheat, He''nan Agricultural University, Zhengzhou 450002, China;National Engineering Research Centre for Wheat, He''nan Agricultural University, Zhengzhou 450002, China;National Engineering Research Centre for Wheat, He''nan Agricultural University, Zhengzhou 450002, China;National Engineering Research Centre for Wheat, He''nan Agricultural University, Zhengzhou 450002, China;National Engineering Research Centre for Wheat, He''nan Agricultural University, Zhengzhou 450002, China |
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| Abstract: | Starch synthase I(SS I) partial cDNA sequences(795 bp)(GenBank No.EF221762) from grains of common wheat(Triticum aestivum,Yujiao 2 cultivar) was amplified by RT-PCR.The result demonstrated that the cloned SS I gene sequences were 84%~98% identified with the reported SS I genes in GenBank previously.In addition,its antisense expression vector was constructed with pWM101,and RNAi vector was also constructed with pFGC5941.These constructed vectors will provide a good background to study the function of SS I on biosynthesis of starch in wheat plants. |
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| Keywords: | Common wheat(Triticum aestivum) Starch synthase I gene Expression vecors |
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