| 两种刺玫花粉离体萌发培养液配方的优化 |
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| 引用本文： | 王泽翻,韩倩,杨恒,李亚齐,曾凡景,孙健,赵惠恩.两种刺玫花粉离体萌发培养液配方的优化[J].湖北农业科学,2012(5):1030-1032. |
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| 作者姓名： | 王泽翻 韩倩 杨恒 李亚齐 曾凡景 孙健 赵惠恩 |
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| 作者单位： | 北京林业大学园林学院 |
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| 摘 要： | 以报春刺玫(Rosa primula)和单瓣黄刺玫(R.xanthina)储存花粉为材料,设计单因素试验和正交试验,考察了蔗糖、硼酸及Ca(NO3)2.4H2O浓度对两种刺玫花粉萌发率的影响。结果表明,适宜浓度的蔗糖和硼酸能促进花粉萌发,而较高浓度的Ca(NO3)2.4H2O会抑制花粉萌发。正交试验结果表明适合报春刺玫花粉萌发的最佳培养基为20%蔗糖(质量分数,下同)+100 mg/L硼酸;适合单瓣黄刺玫花粉萌发的最佳培养基为10%蔗糖+50 mg/L硼酸。
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| 关 键 词： | 报春刺玫(Rosa primula) 单瓣黄刺玫(R.xanthina) 花粉萌发 液体培养基 |
Optimization of Culture Medium Formula for in Vitro Pollen Germination of Rosa primula and R. xanthina |
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| WANG Ze-fan,HAN Qian,YANG Heng,LI Ya-qi,ZENG Fan-jing,SUN Jian,ZHAO Hui-en.Optimization of Culture Medium Formula for in Vitro Pollen Germination of Rosa primula and R. xanthina[J].Hubei Agricultural Sciences,2012(5):1030-1032. |
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| Authors: | WANG Ze-fan HAN Qian YANG Heng LI Ya-qi ZENG Fan-jing SUN Jian ZHAO Hui-en |
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| Institution: | (College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China) |
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| Abstract: | Using stored pollen of Rosa primula and R.xanthina as experimental material,simple factor tests and orthogonal design were applied to evaluate the effects of sucrose,H3BO3 and Ca(NO3)2·4H2O on in vitro pollen germination of two rejuvenation rose.Results showed that sucrose and H3BO3 at proper dose could promote germination of rose pollen;While high concentration of Ca(NO3)2·4H2O could inhibit pollen germination.The optimum culture medium formula for germination of R.primula pollen was 20%+sucrose 100 mg/L H3BO3,and for R.xanthina pollen was 10% sucrose +50 mg/L H3BO3. |
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| Keywords: | Rosa primula R xanthina germination of pollen in vitro germination |
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