| 米根霉HZS6生产高光学纯度L-乳酸发酵条件的研究 |
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| 引用本文： | 张晗星,孟武,封文涛,李十中.米根霉HZS6生产高光学纯度L-乳酸发酵条件的研究[J].山东农业科学,2009(8):66-68. |
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| 作者姓名： | 张晗星 孟武 封文涛 李十中 |
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| 作者单位： | 1. 清华大学核能与新能源研究院,北京,100084
2. 山东轻工业学院,山东,济南,250353 |
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| 基金项目： | 国家“十一五”科技支撑项目(2006BAD07A00)资助 |
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| 摘 要： | 通过研究和控制初始发酵条件，比较产物L-乳酸、D-乳酸组成变化，得出提高L-乳酸光学纯度的米根霉发酵生产条件：在pH6．0、温度34℃、初始葡萄糖浓度100g／L、NH。NO3 2g／L、添加1．5g／L L-乳酸的适合条件下，米根霉HZS6能够发酵生产光学纯度99％以上的L-乳酸，其转化率达到80％，终浓度为81．3g／L。
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| 关 键 词： | L-乳酸 D-乳酸 光学纯度 发酵调控 米根霉 |
Study on Fermentation Conditions for High Optical Purity L-lactic Acid Produced by Rhizopus oryzae |
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| ZHANG Han-xing,MENG Wu,FENG Wen-tao,LI Shi-zhong.Study on Fermentation Conditions for High Optical Purity L-lactic Acid Produced by Rhizopus oryzae[J].Shandong Agricultural Sciences,2009(8):66-68. |
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| Authors: | ZHANG Han-xing MENG Wu FENG Wen-tao LI Shi-zhong |
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| Institution: | 1.Institute of Nuclear and New Energy Technology;Tsinghua University;Beijing 100084;China;2.Shandong Institute of Light Industry;Jinan 250353;China |
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| Abstract: | The effect of fermentation conditions on the optical purity of L-lactic acid produced by Rhizopus oryzae HZS6 was studied.Under the suitable conditions,that is to say,pH 6.0,34℃,100 g/L initial glucose,2 g/L NH4NO3 and 1.5 g/L supplemented L-lactic acid,Rhizopus oryzae HZS6 could produce the L-lactic acid with the optical purity of 99%,the conversion rate of 80% and the final concentration of 81.3 g/L. |
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| Keywords: | L-lactic acid D-lactic acid Optical purity Fermentation control Rhizopus oryzae |
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