桃蚜对噻虫嗪代谢抗性机制研究

对桃蚜进行室内噻虫嗪抗性品系筛选,选育至15代后抗性倍数达到75.6倍。对噻虫嗪敏感品系(THI-S)和抗性品系(THI-R)桃蚜的谷胱甘肽S-转移酶(GSTs)、酸性磷酸酯酶(ACP)、碱性磷酸酯酶(ALP)、羧酸酯酶(CarE)、多功能氧化酶(MFO)O-脱甲基活性进行了比较,结果显示:敏感品系(THI-S)和抗性品系(THI-R)的谷胱甘肽S-转移酶比活力分别为3.127 5和3.215 9,差异不显著,桃蚜抗性品系体内酸性磷酸酯酶、碱性磷酸酯酶、羧酸酯酶和多功能氧化酶O-脱甲基活性均显著高于敏感品系,分别达到了1.57、2.10、6.12、2.03倍。表明桃蚜对噻虫嗪抗性的产生与酸性磷酸酯酶、碱性磷酸酯酶、羧酸酯酶和多功能氧化酶O-脱甲基的活性相关。

Metabolic mechanism of resistance to thiamethoxam in Myzus persicae

Resistance screening of Myzus persicae (Sulzer) to thiamethoxam was conducted in the laboratory. After 15 generations of selection, the resistance index of M. persicae to thiamethoxam was 75.6 folds. The activities of GSTs, ACP, ALP, CarE and MFO O-demethylation were tested in both sensitive (THI-S) and resistant (THI-R) biotypes of M. persicae. The results showed that the activity of GSTs in THI-S and THI-R had no significant difference, with specific activity indexes of 3.127 5 and 3.215 9, respectively. However, the activities of ACP, ALP, CarE and MFO O-demethylation in THI-R were higher than those in THI-S, with indexes of 1.57, 2.10, 6.12 and 2.03, respectively. These results indicated that the resistance of M.persicae to thiamethoxam was related to the activities of ACP, ALP, CarE and MFO O-demethylation.