| 能源林树种乌桕茎段离体培养与快速繁殖 |
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| 引用本文： | 郄亚微,徐有明,李学琴,谭聪敏,胡荣仙.能源林树种乌桕茎段离体培养与快速繁殖[J].东北林业大学学报,2009,37(12). |
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| 作者姓名： | 郄亚微 徐有明 李学琴 谭聪敏 胡荣仙 |
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| 作者单位： | 华中农业大学,武汉,430070 |
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| 基金项目： | 华中农业大学科技处基金资助项目 |
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| 摘 要： | 乌桕是一种经济价值较高的木本油料树种,其种子中的油脂可用于食用油源、工业油源和动力油源,是一种可再生能源培养的优良树种.对乌桕茎段进行离体培养和快速繁殖研究.在初代培养中,先用70%的酒精对带芽茎段消毒30s,再用0.1%升汞灭菌10min,污染率可降到26.5%.在最佳培养基WPM+6-BA 0.5mg/L+NAA 0.05mg/L中进行芽诱导,30d后的诱导率可达62.9%.在MS+6-BA 1.0mg/L+KT 0.5mg/L+NAA 0.5mg/L上进行继代增殖培养,增殖效果最好,增值率为81.2%,增殖倍数为3.2倍.将健壮的无根单芽接种于1/2 MS+IBA 0.5mg/L上进行生根诱导,生根率可达93.3%.
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| 关 键 词： | 乌桕 茎段 离体培养 快速繁殖 |
In Vitro Shoot Culture and Rapid Propagation of Chinese Tallow Trees for Energy Forests |
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| Qie Yawei,Xu Youming,Li Xueqin,Tan Congmin,Hu Rongxian.In Vitro Shoot Culture and Rapid Propagation of Chinese Tallow Trees for Energy Forests[J].Journal of Northeast Forestry University,2009,37(12). |
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| Authors: | Qie Yawei Xu Youming Li Xueqin Tan Congmin Hu Rongxian |
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| Institution: | Qie Yawei,Xu Youming,Li Xueqin,Tan Congmin,Hu Rongxian(College of Horticulture , Forestry,Huazhong Agricultural University,Wuhan 430070,P.R.China) |
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| Abstract: | An experiment was conducted to study in vitro shoot culture and micropropagation of Chinese tallow tree (Sapium sebiferum (L.) Roxb.).Results showed that,in primary culture,the contamination rate reached 26.5% when the explants were disinfected with 70% ethanol for 30 seconds and with 0.1% HgCl2 for 10 minutes.The medium of WPM supplemented with 6-BA 0.5mg/L and NAA 0.05mg/L was excellent for the primary culture,with an induction rate of 62.9%,and MS supplemented with 6-BA 1.0mg/L,KT 0.5mg/L,NAA 0.5mg/L was... |
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| Keywords: | Sapium sebiferum Shoots Culture in vitro Micropropagation |
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