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| 水牡丹的组织培养与快速繁殖 | |
| 引用本文： | 许继勇,麦瑜玲,林书瀚,夏时云.水牡丹的组织培养与快速繁殖[J].天津农业科学,2007,13(1):12-13. |
| 作者姓名： | 许继勇 麦瑜玲 林书瀚 夏时云 |
| 作者单位： | 1. 广东省汕头投资建设总公司,汕头,515041;汕头市中蔬花卉有限公司,汕头515041 2. 汕头市中蔬花卉有限公司,汕头515041 3. 天津市农业生物技术研究中心,天津,300192 |
| 基金项目： | 广东省汕头投资建设总公司项目 |
| 摘 要： | 研究了水牡丹幼嫩花序离体培养中愈伤组织和芽诱导的适宜培养基和6-BA与2,4-D浓度,诱导培养基中6-BA和2,4-D的最佳组合。结果表明:水牡丹幼嫩花序离体培养中愈伤组织和芽诱导以MS为基本培养基,加入2．0 mg·L-16-BA,配合1．0 mg·L-1KT和0．2 mg·L-1NAA效果最好,继代增殖培养以MS为基本培养基,激素以0．5 mg·L-16-BA和1．0 mg·L-1KT, 0．1 mg·L-1NAA时效果较佳。水牡丹离体培养和快繁中无需另作生根培养。但移栽成活率只有60％左右。 |
| 关 键 词： | 水牡丹 幼嫩花序 离体培养 |
| 文章编号： | 1006-6500（2007）01-0012-02 |
| 修稿时间： | 2006-09-05 |
Tissue Culture and Rapid Propagation of Haworthia arachnoidea var. setata |
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| XU Ji-yong,MAI Yu-ling,LIN Shu-han,XIA Shi-yun.Tissue Culture and Rapid Propagation of Haworthia arachnoidea var. setata[J].Tianjin Agricultural Sciences,2007,13(1):12-13. | |
| Authors: | XU Ji-yong MAI Yu-ling LIN Shu-han XIA Shi-yun |
| Institution: | 1 .Shantou Invest Construction General Company, Shantou, Guangdong 515041, China; 2.Shantou Zhongshu Flower Company Ltd., Shantou, Guang- dong 515041, China ; 3.Tianjin Agricultural Biotechnology Research Center, Tianjin 300192, China |
| Abstract: | In this study, a protocol for plant regeneration from inflorescence pieces was established. It involved the culture of 1~2 cm long pieces of inflorescence stem on MS medium, 30.0 g·L-1 sucrose, 7.0 g·L-1 agar, 1.0 mg·L-1 KT, 2.0 mg·L-1 6-BA and 0.2 mg·L-1 NAA. The callus were transferred to fresh medium with 0.5 mg·L-1 6-BA, 1.0 mg·L-1KT, 0.1 mg·L-1NAA, where bulblet developed. It had no necessary for root induction. However the survival rate of plants transplanted on a large scale was only 60%. |
| Keywords: | Haworthia arachnoidea var setata immature inflorescence in vitro culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |