Determination of organic acids in tissues and exudates of maize, lupin, and chickpea by high-performance liquid chromatography-tandem mass spectrometry |
| |
Authors: | Erro Javier Zamarreño Angel M Yvin Jean-Claude Garcia-Mina Jose M |
| |
Affiliation: | Department of Chemistry and Soil Chemistry, University of Navarra, 31080 Pamplona, Spain. |
| |
Abstract: | This article describes a fast and simple methodology for the extraction and determination of organic acids in tissues and root exudates of maize, lupin, and chickpea by LC/MS/MS. Its main advantage is that it does not require sample prepurification before HPLC analysis or sample derivatization to improve sensibility. The results obtained showed good precision and accuracy, a recovery close to 100%, and no significant matrix effect. Moreover, the sensibility of the method is in general better than that of previously described methodologies, with detection limits between 15 and 900 pg injected. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|