中国水仙上一种球形病毒的研究Ⅰ.病毒分离及其生物学特性

 从水仙黄条病株上分离到一种球形病毒,直径约21nm,汁液摩擦接种35科(属)55(品)种植物,可侵染其中5科15(品)种,主要局限于豆种(Leguminosae)、藜科(Chenopodiaceae)以及茄科(Solanaceae)、苋科(Amaranthaceae)与番杏科(Aizoaceae)的个别种。在多数寄主上诱导局部坏死,豆科寄主上还伴随叶脉坏死症状产生。其中,昆诺阿藜(Chenopodium quinoa)、苋色藜(C.amaranticolor)、千日红(Gompherena globosa)、番杏(Tetragonia expansa)、大豆(Glycine max)、豇豆(Vigna sinensis)、克利夫兰烟(Nicotiana clevelandii)可作为诊断寄主;千日红、番杏与三叶草(Trifolium sibth)可作为毒源保存寄主。#br#在昆诺阿藜叶片汁液中,该病毒分离物的体外存活期在60天以上(25℃),热钝化点80-85℃(10分钟),稀释限点10^-5-10^-6。回接水仙健株,诱导产生系统褪绿条纹症状,不诱导水仙叶片产生具有特殊结构的内含体。

A SPHERICAL VIRUS ISOLATED FROM NARCISSUS TAZETTA VAR. CHINESIS 1. Isolation of the virus and its biological properties

A spherical virus about 21nm in diameter was isolated from the diseased plants of N.tazetta var.chinesis showing yellow stripe in China. This virus isolate infected 15 species and varieties belonging to 35 families.By mechanical sap inoculation, the virus infection usually resulted in the development of necrotic lessions on the inoculated leaves of Chenopodiaceae, Leguminosae and some members of Amaranthaceae, Aizoaceae and Solanaceae.In the case of most infected plants of Leguminosae, the symptoms of necrotic lessions were usually followed by leaf vein necrosis. However besides the chlorotic stripe on the leaves of narcissus after back inoculation, it rarely produced systemic symptoms on other host plants. In the present studies, Chenopodia quinoa, C. amaranticolor,Glycine max, Uigna sinensis, Gomphrena globosa, Tetragonia expansa and Nicotiana clevelandii were generally used as a set of diagnosis host plants.The last named three species were also used to propagate and maintain the virus. In C.quinoa sap,TIP was 80-85℃,the DEP was at 10^-5-10^-6 and its longevity at 25℃ was 2 months. By electron microscopy, no specific inclusion bodies associated with the virus infection had been found in narcissus leaves.