油茶扦插生根过程的生理生化基础研究

以湘林系列油茶Camellia oleifera品种XL1与XL4插穗为材料，研究了其扦插生根过程中可溶性蛋白、可溶性糖及过氧化物酶（POD）活性变化。结果表明:①油茶属于愈合组织生根类型；②XL1可溶性蛋白及可溶性糖在整个生根过程中始终高于XL4，可溶性蛋白均为N型变化趋势，可溶性糖呈现先上升后下降的趋势，两者的积累为不定根的形成提供营养物质；③XL4的POD活性始终大于XL1，POD活性在愈合组织形成期和不定根诱导期出现高峰，利于根原基的诱导；POD活性在愈合组织和不定根表达期下降，利于根原基发育及不定根的伸长。图4表1参20

Physiological and biochemical characteristics of Camellia oleifera during root cutting establishment

The aim of this study was to determine the soluble protein content，soluble sugar content，and peroxidase（POD）activity for two Camellia oleifera cultivars（XL1 and XL4）during root cutting establishment. The content of soluble proteins was detected by coomassie brilliant blue method，soluble sugar content was detected by anthrone colorimetry，and the POD activity was detected by guaiacol method. Results showed that rooting of C. oleifera was derived from the callus. Also，during root cutting establishment the content of soluble proteins and soluble sugars for XL1 was higher than XL4，and soluble protein content increased first，decreased later，and then increased rapidly. Soluble sugar content first increased and then decreased. Additionally，POD activity of XL4 was higher than XL1 reaching a peak during the callus formation phase and the adventitious root induction phase，which was beneficial to the induction of root primordia. POD activity decreased during the expression period of callus and adventitious roots，which was beneficial to the growth of root primordia and elongation of adventitious roots. ［Ch， 4 fig. 1 tab. 20 ref.］