<Emphasis Type="Italic">In vitro</Emphasis> chemosensitivity of canine mast cell tumors grades II and III to all-<Emphasis Type="Italic">trans</Emphasis>-retinoic acid (ATRA). |
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Authors: | K C Pinello M Nagamine T C Silva P Matsuzaki H V Caetano L N Torres H Fukumasu J L Avanzo J M Matera M L Z Dagli |
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Institution: | 1.Laboratory of Experimental Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science,University of S?o Paulo,S?o Paulo,Brazil;2.Department of Surgery, School of Veterinary Medicine and Animal Science,University of S?o Paulo,S?o Paulo,Brazil |
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Abstract: | Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect the skin and soft tissue of dogs. Because mast cell
tumors present a great variety of clinical appearance and behavior, their treatment becomes a challenge. While retinoids are
well recognized as promising antitumor agents, there have been only a few reports about retinoids’ effect on canine cancers.
The aim of this study was to investigate the chemosensitivity of MCT grades II and III to all-trans retinoic acid (ATRA).
Immediately after surgical resection, MCT were prepared for primary culture. Samples of MCTs were also fixed in formalin for
histopathology and grading according to the classification of Patnaik et al. (Veterinary Pathology 21(5):469–474, 1984). The best results were obtained when neoplastic mast cells were co-cultivated with fibroblasts. Cultured mast cells were,
then, treated with concentrations of 10−4 to 10−7 M of ATRA, in order to evaluate their chemosensitivity to this retinoid. MTT assay was performed to estimate cell growth
and death. The highest level of mast cell chemosensivity was obtained at the dose of 10−4 M (p < 0,002). MCT of grades II or III were equally susceptible to the treatment with ATRA. Cell death was observed on the
first 24 h until 48 h. According to these results, ATRA may be a potential chemotherapeutic agent for the treatment of canine
MCT. |
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Keywords: | Canine mast cell tumor Primary cell culture Chemosensitivity Retinoic acid ATRA |
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