不同萃取头萃取萝卜风味物质的GC-MS分析

采用PDMS/DVB和DVB/CAR/PDMS两种萃取头,以固相微萃取(SPME)法提取萝卜风味物质成分,经气相色谱-质谱联用(GC-MS)对风味物质成分进行鉴定,分析比较两种萃取头提取的风味物质成分差别,以确定适宜的萃取头。结果表明:两种萃取头的固相微萃取共检测到29种风味成分,PDMS/DVB提取数量为17种,DVB/CAR/PDMS为27种;PDMS/DVB萃取头所萃取的醇类、异硫氰酸酯类的数量比DVB/CAR/PDMS萃取头少2种,烷烃类少3种,杂环类少1种,酯类、酮类均未检测到;DVB/CAR/PDMS萃取异硫氰酸酯类、烷烃类、醛类、杂环类、酮类、醚类及醇类的灵敏度高,PDMS/DVB萃取芳香族类的灵敏度高。因此,根据获得风味物质数量、种类以及灵敏度,供试萃取头中,DVB/CAR/PDMS比PDMS/DVB更适于提取分析萝卜风味物质成分。

GC-MS Analysis of Flavor Compounds in Radish Extracted by Different Extraction Heads

Two extraction heads, PDMS/DVB and DVB/CAR/PDMS, were used to extract the flavor compounds in radish by solid phase microextraction （SPME） method.To determine appropriate extraction head, the flavor compounds were detected by chromatography -mass spectrometry （ GC -MS） method and their differences were compared .The results showed that 29 kinds of flavor compounds in total were detected using two extraction heads .Seventeen were extracted by PDMS/DVB, and 27 were extracted by DVB/CAR/PDMS.The quantity of flavor compounds extracted by PDMS/DVB was less than that by DVB/CAR/PDMS, including 2 kinds of alcohols and isothiocyanates , 3 kinds of alkanes , 1 kind of heterocyclics .Lipids and ke-tones were not detected by PDMS/DVB.DVB/CAR/PDMS had higher sensitivity in extracting isothiocya-nates, alkanes, aldehydes, heterocyclics, ketones, ethers and alcohols, while PDMS/DVB was more sensitive in extracting aromatic compounds .According to the quantity and kind of flavor compounds and sensitivity , DVB/CAR/PDMS was more suitable for extracting flavor compounds in radish than PDMS /DVB.