| 草菇液体菌种培养条件优化研究 |
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| 引用本文： | 任海霞,杨鹏,曲玲,任鹏飞,李瑾,宫志远.草菇液体菌种培养条件优化研究[J].山东农业科学,2014(1):58-60. |
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| 作者姓名： | 任海霞 杨鹏 曲玲 任鹏飞 李瑾 宫志远 |
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| 作者单位： | 山东省农业科学院农业资源与环境研究所,山东济南250100 |
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| 基金项目： | 基金项目：山东省良种工程项目“山东设施名产蔬菜及食用菌新品种选育”（2011LZ006-04） |
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| 摘 要： | 为了完善草菇液体菌种的生产和应用,尽快使草菇实现工厂化栽培,本试验以天达V901为试材,通过摇瓶发酵,进行了草菇液体菌种培养条件优化研究。结果表明,液体培养条件下,适宜草菇菌丝生长的最佳碳源是淀粉,最佳氮源是蛋白胨,最佳碳氮比是10∶1,最佳淀粉浓度是3.0%。草菇菌丝生长的最适温度为30℃,最适初始pH为7.0。
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| 关 键 词： | 草菇 液体培养 营养物质 培养条件 优化试验 |
Optimization of Liquid Culture Conditions for Volvariella volvacea |
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| Ren Haixia,Yang Peng,Qu Ling,Ren Pengfei,Li Jin,Gong Zhiyuan.Optimization of Liquid Culture Conditions for Volvariella volvacea[J].Shandong Agricultural Sciences,2014(1):58-60. |
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| Authors: | Ren Haixia Yang Peng Qu Ling Ren Pengfei Li Jin Gong Zhiyuan |
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| Institution: | Ren Haixia, Yang Peng, Qu Ling, Ren Pengfei, Li Jin, Gong Zhiyuan |
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| Abstract: | In order to improve the production and application of liquid Volvariella volvacea strains and as soon as possible to achieve its industrial cultivation , the suitable submerged nutrient conditions and liquid cul-ture conditions for Volvariella volvacea strain V901 were studied by flask shaking in this paper .The results showed that the optimal carbon and nitrogen sources were starch and peptone in the liquid culture .The best C/N ratio was 10∶1 , and the best starch concentration was 3 .0%.The suitable culture temperature and initial pH value were 30℃and 7 .0 respectively for mycelia growth . |
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| Keywords: | Volvariella volvacea Liquid culture Nutrient Culture condition Optimization test |
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