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| 消化道上皮细胞中乳酸杆菌黏附配体蛋白含量的变化 | |
| 引用本文： | 林雪彦,王中华,牛钟相. 消化道上皮细胞中乳酸杆菌黏附配体蛋白含量的变化[J]. 中国兽医学报, 2012, 32(3): 393-396 |
| 作者姓名： | 林雪彦 王中华 牛钟相 |
| 作者单位： | 山东农业大学动物科技学院,山东泰安,271018 |
| 基金项目： | 山东省自然科学基金资助项目(Y2008D28);国家现代农业产业技术体系建设资助项目(nycytx-10) |
| 摘 要： | 利用兔抗鸡乳酸杆菌黏附配体蛋白血清,以不连续活性-PAGE电泳技术和间接ELISA方法,对不同日龄、健康和患球虫病鸡消化道不同部位乳酸杆菌黏附配体蛋白含量进行了检测。结果表明,2日龄鸡嗉囊部位产生乳酸杆菌黏附配体蛋白成分,D450nm值为0.181;1日龄鸡小肠部位产生乳酸杆菌黏附配体蛋白成分,D450nm值为0.168;5日龄乳酸杆菌黏附配体蛋白成分达到稳定,D450nm值分别为0.200和0.123。健康鸡体内嗉囊与小肠部位乳酸杆菌黏附配体蛋白含量比患球虫病鸡明显增多,D450nm值分别为：嗉囊,0.143和0.132;小肠,0.148和0.134。 |
| 关 键 词： | 消化道上皮细胞 乳酸杆菌 配体蛋白 |
Variation on Lactobacillus adhesion match protein content of digestive tract epithelial cells |
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| LIN Xue-yan,WANG Zhong-hua,NIU Zhong-xiang. Variation on Lactobacillus adhesion match protein content of digestive tract epithelial cells[J]. Chinese Journal of Veterinary Science, 2012, 32(3): 393-396 | |
| Authors: | LIN Xue-yan WANG Zhong-hua NIU Zhong-xiang |
| Affiliation: | (College of Animal Science and Technology,Shandong Agricultural University,Tai′an,Shandong 271018,China) |
| Abstract: | Use of antiserum of hare resist chicken digestive tract epithelial cells Lactobacillus adhesion match protein,by discontinuation alive-PAGE electrophoresis and indirected ELISA technology,detected Lactobacillus adhesion match protein content in crop and intestine of 1-40-day-old healthy and coccidiosis chicken.The results suggested that 2-day-old chicken crops have Lactobacillus adhesion receptor protein,D450 nm is 0.181,1-day-old chicken intestines have Lactobacillus adhesion receptor protein,D450 nm is 0.168.Its stabilization is at 5 day,D450 nm is 0.200 and 0.123 respectively.Healthy chicken crop and intestines have more Lactobacillus adhesion match protein than coccidiosis chicken.D450 nm is 0.143,0.132 in crop;0.148,0.134 in intestines respectively. |
| Keywords: | digestive tract intestinal epithelial cells Lactobacillus adhesion match protein |
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