穿龙薯蓣新陈种子萌发条件优选研究

目的:研究赤霉素( GA3)、萘乙酸(NAA)和层积后温度对穿龙薯蓣新陈种子萌发条件.方法:培养皿培养法遮光培养,得出3种处理最优条件后对陈种子进行相同处理.结果:穿龙薯蓣新种子4℃低温砂层积后,25℃条件下发芽率和发芽势最高,分别为80％和53.33％,陈种子为45％和25％；而采用100 mg/L GA3直接浸泡未层积新种子24h,发芽率和发芽势可达66.65％和48.35％,陈种子为40％和25％；1 mg/L NAA浸泡12 h新种子发芽率和发芽势为58.35％和43.35％,陈种子为30％和23.35％；低温砂层积新种子发芽进程最快,其第6天开始发芽,第12天发芽完毕,快于GA3的10d和22 d,NAA的11d和24d.结论:生产上若有层积条件,建议采用4℃低温砂层积60d后在25℃下进行新种子催芽；若没有层积条件,建议直接采用100 mg/L GA3进行新种子催芽处理.

Study on the Optimum Condition of New and Old Seed Germination of Dioscorea nipponica Makino

Objective:The author studied the optimization of GA3、NAA and cold sandy stratification treatment on new and old seeds germination of Dioscorea nipponica Makino.Methods:The research used the method of culture,and the best condition that was selected from 3 kinds of treatments on old seeds.Results The result showed that the D.nipponica new seeds just treated with cold sandy stratification(4 ℃) germinated best at 25 ℃,and the germination rate and germination energy were 80% and 53.33%,and the old seeds’ were 45% and 25% respectively.The new seeds treated with 100 mg/L GA3 for 24 hours germinated the best,and the germination rate and germination energy were 66.65% and 48.35%,and the old seeds’ were 40% and 25% respectively.The new seeds treated with 1 mg/L NAA for 12 hours germinated the best and the germination rate and germination energy were 58.35% and 43.35%,and the old seeds’ were 30% and 23.35% respectively.The new seeds treated with 100 mg/L GA3 for 24 hours began to germinate on the 10th day and finished on the 22 nd day,and the new seeds treated with 1 mg/L NAA for 12 hours began to germinate on the 11 th day and finished on the 24th day.The new seeds treated with cold sandy stratification(4 ℃) began to germinate on the 6th day and finished on the 12th day.Conclusion:If cold sandy stratification would be available in production process,it could be recommended to treat D.nipponica new seeds with cold sandy stratification(4 ℃) for 60 days and then accelerate germina at 25 ℃.If not,treat D.nipponica new seeds with 100 mg/L GA3 for 24 hours directly.