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4个茶树品种自交后代群体遗传结构分析
引用本文:杨军,王让剑,孔祥瑞,郑国华.4个茶树品种自交后代群体遗传结构分析[J].茶叶科学简报,2016(2):59-63.
作者姓名:杨军  王让剑  孔祥瑞  郑国华
作者单位:福建省农业科学院茶叶研究所/国家茶树改良中心福建分中心,福建 福安,355015
基金项目:福建省现代农业(茶叶)产业技术体系(2014~2016);农业部福建茶树及乌龙茶加工科学观测实验站(2011~2015)。
摘    要:利用 EST-SSR 对茶树品种紫玫瑰、金牡丹、茗科1号(金观音)、悦茗香及其自然杂交后代,进行亲缘关系、遗传多样性、群体结构分析。16对 EST-SSR 标记共检测到等位位点125个,反映位点多态性水平的PIC 值平均值为0.617,变幅为0.159~0.840。参试材料的观测杂合度、期望杂合度、Nei′s 基因多样性、Shannon 信息多样性指数的平均值分别为0.579、0.639、0.632、1.281。结果表明,参试自然杂交后代有较强母本遗传效应,4个群体具有明显的群体遗传组成特征,Structure 软件适宜茶树品种群体遗传结构分析。

关 键 词:茶树  自然杂交  EST-SSR  亲缘关系  群体结构

Genetic Structure of Natural Hybrids of Four Tea Varieties
YANG Jun,WANG Rang-jian,KONG Xiang-rui,ZHENG Guo-hua.Genetic Structure of Natural Hybrids of Four Tea Varieties[J].Tea Science and Technology,2016(2):59-63.
Authors:YANG Jun  WANG Rang-jian  KONG Xiang-rui  ZHENG Guo-hua
Abstract:EST-SSR was used to analyze the genetic polymorphism,relationship,and population structure of Zimeigui,Jinmudan,Mingke 1 (Jinguanyin),Yuemingxiang,and the natural hybrids of Zimeigui,Jinmudan, Jinguanyin,and Yuemingxiang. A total of 125 alleles were amplified using 16 pairs of EST-SSR primers. The average PIC was 0.617, varying from 0.159 to 0.840. The average observed heterozygosity, expected heterozygosity,Nei′s genetic diversity,Shannon information diversity index among them were 0.579,0.639, 0.632,and 1.281,respectively. A strong maternal parental effect seemed apparent as shown by the genetics of the natural hybrids. The population structures of the 4 groups had distinctive genetic characteristics. It was concluded that the software applied could be adequately used for analyzing the genetic structures of tea populations.
Keywords:tea plant  natural hybrids  EST-SSR  genetic relationship  population structure
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