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| 石龟嗜水气单孢菌的分离鉴定及药敏特性研究 | |
| 引用本文： | 华俊,谢彬,黄红梅,袁书智,白安斌,吴健敏.石龟嗜水气单孢菌的分离鉴定及药敏特性研究[J].广西农业科学,2010,41(3):273-276. |
| 作者姓名： | 华俊 谢彬 黄红梅 袁书智 白安斌 吴健敏 |
| 作者单位： | 1. 广西兽医研究所,南宁,530001 2. 广西兽医研究所,南宁,530001;惠朋动物药业有限责任公司,南宁,530001 3. 惠朋动物药业有限责任公司,南宁,530001 |
| 基金项目： | 广西大型仪器协作共用网服务体系建设与示范项目(08-05-01D);;广西兽医研究所基本科研业务费专项项目(桂兽研专项08-4) |
| 摘 要： | 从患病石龟上分离获得1株革兰氏阴性杆菌，菌体细长、无芽孢和荚膜，在山羊血琼脂平板上能形成明显的β-溶血圈。经小白鼠致病性试验、生理生化鉴定及16SrRNA序列分析，确定该分离株为致病性嗜水气单孢菌。经同源性及遗传进化分析发现，该菌株与嗜水气单孢菌B27、HSO2（Genbank登录号：FJ494900．1、FJ562211．1）同源性高达99．5％。而药敏试验表明，该分离株除了对赛福欣（复方恩诺沙星）、肠清（复方乳酸环丙沙星）2种药物高度敏感外，对大部分抗菌素均不敏感。 |
| 关 键 词： | 石龟 嗜水气单孢菌 分离鉴定 16S rRNA 药敏特性 |
Isolation and identification of Aeromonas hydrophila from Mauremys mutica Cantor and its antibiotic sensitivity |
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| HUA Jun,XIE Bin,HUANG Hong-mei,YUAN Shu-zhi,BAI An-bin,WU Jian-min.Isolation and identification of Aeromonas hydrophila from Mauremys mutica Cantor and its antibiotic sensitivity[J].Guangxi Agricultural Sciences,2010,41(3):273-276. | |
| Authors: | HUA Jun XIE Bin HUANG Hong-mei YUAN Shu-zhi BAI An-bin WU Jian-min |
| Institution: | 1 Guangxi Veterinary Research Institute;Nanning 530001;China;2 Huipeng Animal Drugs Co.Ltd.;Nanning;Guangxi 530001;China |
| Abstract: | The secretions of throat, liver and lungs were sampled from young diseased Mauremys mutica Cantor, from Wuming, Nanning, Guangxi. One gram negative bacilli was isolated which was without spore and capsule, having gracile thallus and showed β-haemolysis on SBA. Pathogenicity tests, physiological and biochemical characteristics identification, antibiotic sensitivity test and sequence analysis of 16S rRNA showed that it was a strain of Aeromonas hydrophila （08- 228-1R）. The homology and inheritance evolution analysis of 16S rRNA showed 99.5% homology of this strain with Aeromonas hydrophila strains B27 and HSO2 （locus number： FJ494900.1 and FJ562211.1）. In addition, the strain was highly sensitive to Saifuxing （Compound Enrofloxacin）, Changqing （Compound Ciprofloxacin lactate）, but insensitive to other antibiotics. |
| Keywords: | Mauremys mutica Cantor Aeromonas hydrophila isolation and identification 16S rRNA antibiotic sensitivity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |