| 影响油棕叶片愈伤组织诱导因素研究 |
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| 引用本文： | 邹积鑫,尤丽莉,林位夫. 影响油棕叶片愈伤组织诱导因素研究[J]. 热带农业科学, 2014, 0(2): 54-58,68 |
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| 作者姓名： | 邹积鑫 尤丽莉 林位夫 |
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| 作者单位： | [1]中国热带农业科学院橡胶研究所,海南儋州571737 [2]海南大学,海南海口571737 |
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| 基金项目： | 基金项目：中国热带农业科学院橡胶研究所基本科研业务费专项“油棕叶片的组织培养”（No.YWFZX2010-09）：“油棕次生胚状体的诱导及植株再生体系的建立”（No.1630022012004）. |
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| 摘 要： | 研究不同取材部位、激素组合配比等因素对3个不同油棕品系叶片愈伤组织培养的影响。结果表明:不同部位的油棕叶片以及不同品系的油棕愈伤组织诱导存在极显著差异;试验以MS培养基+5 mg/L 2,4-D+10 mg/L TDZ+6 g/L琼脂+30 g/L糖配比获得较好的愈伤诱导率;添加2%活性炭可以有效抑制油棕叶片愈伤组织褐化。
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| 关 键 词： | 油棕 叶片 愈伤组织 |
Some Factors Affecting Callus Induction from Oil Palm Leaves |
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| ZOU Jixin,YOU Lili,LIN Weifu. Some Factors Affecting Callus Induction from Oil Palm Leaves[J]. Chinese Journal of Tropical Agriculture, 2014, 0(2): 54-58,68 |
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| Authors: | ZOU Jixin YOU Lili LIN Weifu |
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| Affiliation: | 1 Rubber Research Institute, CATAS, Danzhou, Hainan 571737; 2 Hainan University, Haikou, Hainan 570228) |
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| Abstract: | Different parts of leaves and hormone combinations involved in oil palm leave callus culture for three strains of oil palm were studied. The result indicated that different parts of leaves and different strains of oil palm have significant difference in callus induction. The optimum medium components for oil palm callus induction were Murashige-Skoog （MS） medium supplemented with 5 mg-L-12, 4-D and 10 mg/L TDZ and 6 g/L agar and 30 g/L sucrose. The supplying of 2% charcoal can prevent explants from browning efficiently. |
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| Keywords: | oil palm leaves callus |
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